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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

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Caspase-3 gene transfected with LIGHT gene: can it be used for therapy of human hepatocellular carcinoma?

Yun Lu1 / Li-Qun Wu2 / Shou-Guang Wang3 / Zhen-Hua Lv4 / Bing Han5

1Department of Hepatobiliary Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China

2Department of Hepatobiliary Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China

3Center of Cell and Molecular Pathology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China

4Center of Cell and Molecular Pathology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China

5Department of Hepatobiliary Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China

Corresponding author: Yun Lu, Associate Professor, Department of Hepatobiliary Surgery, Affiliated Hospital of Medical College, Qingdao University, No. 16 Jiangsu Rd., Qingdao 266003, Shandong Province, China Phone: +86-532-82911369, Fax: +86-532-82911999,

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 46, Issue 4, Pages 470–474, ISSN (Online) 14374331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2008.094, February 2008

Publication History

Received:
2007-10-20
Accepted:
2007-11-30
Published Online:
2008-02-26

Abstract

Background: The aim of this study was to detect the expression of apoptosis factor caspase-3 in transferred HepG2 cells and provide feasible evaluation of the treatment for primary liver cancer with gene methods.

Methods: The pcDNA4C-LIGHT cDNA was extracted from Escherichia coli JM-109; then, the pcDNA4C-LIGHT cDNA was transferred into the HepG2 cells by a cationic liposome mediated method. Meanwhile, the blank group was established as the control group and the HepG2 cells were collected after transfection at 12 h, 24 h, 48 h, 3 days and 5 days. The expression of caspase-3 was identified in the supernatants by ELISA. A standard curve was generated for the set of samples assayed. Statistical significance was analyzed by SPSS.

Results: The quantity of caspase-3 protein was the greatest at 48 h and the least on day 5. The secretion of caspase-3 did not increase in the control group. The coefficient of correlation was equal to 0.9986 and had evident significance.

Conclusions: The pcDNA4C-LIGHT was effectively transfected in human HepG2 cells mediated by liposome. The expression of caspase-3 increased in the transfected group. This study provides necessary theoretic support for the treatment of liver cancer with gene methods.

Clin Chem Lab Med 2008;46:470–4.

Keywords: caspase-3; hepatocellular carcinoma; HepG2 cell; LIGHT gene; transfection

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