Jump to ContentJump to Main Navigation

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.

12 Issues per year


IMPACT FACTOR 2014: 2.707
Rank 6 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR): 0.860
Source Normalized Impact per Paper (SNIP): 1.046

VolumeIssuePage

Issues

Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems

Vratislav Chromý1 / Kateřina Rozkošná2 / Pavel Sedlák3

1Institute of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic

2Diagnostics Research Department, Pliva-Lachema Diagnostika, Brno, Czech Republic

3Department of Clinical Biochemistry, Municipal Hospital, Čáslav, Czech Republic

Corresponding author: Prof. Dr. Vratislav Chromý, Institute of Chemistry, Faculty of Science, Masaryk University, Komenského 2, Brno, Czech Republic Phone: +420-549494555,

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 46, Issue 8, Pages 1127–1133, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2008.224, August 2008

Publication History

Received:
2008-02-18
Accepted:
2008-04-10

Abstract

Background: The calibration of Jaffe method for serum creatinine using one serum-based standard complemented with artificial matrix compensating standard's Jaffe-interfering substances allows two-point calibration with results well comparable with enzymatic methods.

Method: Spectrophotometry was used.

Results: Jaffe procedures compensating serum/plasma intereferents by subtracting a constant amount of creatinine poorly overcompensate creatinine in children. Two-point calibration with a pair of primary serum standards certified by the reference measurement procedure (isotope-dilution, mass spectrometry) or with a pair of secondary standards linked to primary materials could provide results well agreeable with enzymatic determination. Such a calibration comprises an absorbance offset corresponding to other-than-creatinine Jaffe-interfering chromogens present in standards in the calibration line, while a two-point calibration combining one standard with physiological saline/water always grossly distorts calibration line. We calculated/prepared artificial serum matrices capable of compensating Jaffe-interfering chromogens in serum standards. The combination of even one standard with its artificial matrix also enables two-point calibration with practically the same results as with a pair of primary standards.

Conclusions: A two-point calibration of Jaffe method for serum creatinine combining only one serum standard with creatinine solution matching standard's allow matrix-present interferents present results well comparable with enzymatic determination, providing the standard is attested/linked to the reference measurement procedure.

Clin Chem Lab Med 2008;46:1127–33.

Keywords: artificial serum matrix for calibration of Jaffe method; compensation of serum interferents in Jaffe method; Jaffe serum creatinine

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Lucie Sancey, Shady Kotb, Charles Truillet, Florence Appaix, Arthur Marais, Eloïse Thomas, Boudewijn van der Sanden, Jean-Philippe Klein, Blandine Laurent, Michèle Cottier, Rodolphe Antoine, Philippe Dugourd, Gérard Panczer, François Lux, Pascal Perriat, Vincent Motto-Ros, and Olivier Tillement
ACS Nano, 2015, Volume 9, Number 3, Page 2477
[2]
Mohamed A. Dkhil, Saleh Al-Quraishy, Marwa M.S. Diab, Mohamed S. Othman, Ahmed M. Aref, and Ahmed E. Abdel Moneim
Food and Chemical Toxicology, 2014, Volume 74, Page 98
[3]
Fernando J. Hermida, María J. Lorenzo, Aida Pérez, María Fernández, Olatz Sagastagoia, and Concepción Magadán
Scandinavian Journal of Clinical & Laboratory Investigation, 2014, Volume 74, Number 7, Page 629
[4]
Mohamed S. Othman, Gehan Safwat, Menna Aboulkhair, and Ahmed E. Abdel Moneim
Food and Chemical Toxicology, 2014, Volume 69, Page 175
[5]
Joana Guimarães, Maria Augusta Vieira-Coelho, Eduardo Moura, Maria José Rosas, Rui Vaz, and Carolina Garrett
Parkinsonism & Related Disorders, 2013, Volume 19, Number 9, Page 836
[6]
William R. de Araújo, Maiara O. Salles, and Thiago R.L.C. Paixão
Sensors and Actuators B: Chemical, 2012, Volume 173, Page 847
[7]
Farhad Arzideh, Werner Wosniok, and Rainer Haeckel
Clinica Chimica Acta, 2010, Volume 411, Number 3-4, Page 215
[8]
Hassan Yankuzo, Qamar Uddin Ahmed, Rahajoe Imam Santosa, Seikh Farid Uddin Akter, and Norlewati A. Talib
Journal of Ethnopharmacology, 2011, Volume 135, Number 1, Page 88
[9]
Vanda Pinto, João Amaral, Elisabete Silva, Sónia Simão, José Miguel Cabral, Joana Afonso, Maria Paula Serrão, Pedro Gomes, Maria João Pinho, and Patrício Soares-da-Silva
Mechanisms of Ageing and Development, 2011, Volume 132, Number 6-7, Page 298
[10]
Zlata Flegar-Meštrić, Sonja Perkov, Barbara Šimonović, and Dubravka Juretić
Clinical Chemistry and Laboratory Medicine, 2010, Volume 48, Number 2
[11]
Edward Goucher, Andrew Kicman, Kim Wolff, Norman Smith, and Sue Jickells
Journal of Separation Science, 2010, Volume 33, Number 6-7, Page 955

Comments (0)

Please log in or register to comment.