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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Editorial Board Member: Gillery, Philippe / Kazmierczak, Steven / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Whitfield, John B.

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Determination of serum creatinine by Jaffe method and how to calibrate to eliminate matrix interference problems

Vratislav Chromý1 / Kateřina Rozkošná2 / Pavel Sedlák3

1Institute of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic

2Diagnostics Research Department, Pliva-Lachema Diagnostika, Brno, Czech Republic

3Department of Clinical Biochemistry, Municipal Hospital, Čáslav, Czech Republic

Corresponding author: Prof. Dr. Vratislav Chromý, Institute of Chemistry, Faculty of Science, Masaryk University, Komenského 2, Brno, Czech Republic Phone: +420-549494555,

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 46, Issue 8, Pages 1127–1133, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2008.224, August 2008

Publication History:
Received:
2008-02-18
Accepted:
2008-04-10

Abstract

Background: The calibration of Jaffe method for serum creatinine using one serum-based standard complemented with artificial matrix compensating standard's Jaffe-interfering substances allows two-point calibration with results well comparable with enzymatic methods.

Method: Spectrophotometry was used.

Results: Jaffe procedures compensating serum/plasma intereferents by subtracting a constant amount of creatinine poorly overcompensate creatinine in children. Two-point calibration with a pair of primary serum standards certified by the reference measurement procedure (isotope-dilution, mass spectrometry) or with a pair of secondary standards linked to primary materials could provide results well agreeable with enzymatic determination. Such a calibration comprises an absorbance offset corresponding to other-than-creatinine Jaffe-interfering chromogens present in standards in the calibration line, while a two-point calibration combining one standard with physiological saline/water always grossly distorts calibration line. We calculated/prepared artificial serum matrices capable of compensating Jaffe-interfering chromogens in serum standards. The combination of even one standard with its artificial matrix also enables two-point calibration with practically the same results as with a pair of primary standards.

Conclusions: A two-point calibration of Jaffe method for serum creatinine combining only one serum standard with creatinine solution matching standard's allow matrix-present interferents present results well comparable with enzymatic determination, providing the standard is attested/linked to the reference measurement procedure.

Clin Chem Lab Med 2008;46:1127–33.

Keywords: artificial serum matrix for calibration of Jaffe method; compensation of serum interferents in Jaffe method; Jaffe serum creatinine

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