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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.

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Validation for quantification of immunoglobulins by Fourier transform infrared spectrometry

Lamia Benezzeddine-Boussaidi1 / Georges Cazorla2 / Anne-Marie Melin3

1Centre National de Médecine et des Sciences du Sport, Unité de recherche Evaluation Sport Santé, Tunis, Tunisia

2Université Victor Segalen Bordeaux 2, Faculté des Sciences du Sport et de l'Education Physique, Laboratoire Evaluation Sport Santé, Bordeaux, France

3Université Victor Segalen Bordeaux 2, INSERM U577, Bordeaux, France

Corresponding author: Georges Cazorla, Université Victor Segalen Bordeaux 2, Faculté des Sciences du Sport et de l'Éducation Physique, Laboratoire Evaluation Sport Santé, 12 Avenue Camille Julian, 33607 Pessac, France Phone: +33-5-56845227, Fax: +33-5-56845235,

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 47, Issue 1, Pages 83–90, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2009.007, December 2008

Publication History

Received:
2008-04-30
Accepted:
2008-09-01
Published Online:
2008-12-31

Abstract

Background: The objective of this study was to develop a robust quantification method for simultaneously analyzing molecules in human plasma using the Fourier transform infrared (FT-IR) system with a partial least square (PLS) regression.

Methods: Plasma spectra were analyzed from 4000 to 500 cm−1 (with 2.0 cm−1 of resolution and 32 scans), and the molecule concentrations (IgA, IgG, IgM) were measured blindly by using a cross-validation model prepared by PLS analysis of data from 135 samples.

Results: There was a significant correlation between the FT-IR predicted concentration and the concentration obtained with the clinical reference method: R2=0.98 (IgA), R2=0.98 (IgG), and R2=0.97 (IgM). The root mean square error of prediction (RMSEP) was 0.05 g·L−1 (IgA), 0.4 g·L−1 (IgG), and 0.03 g·L−1 (IgM). Variability of inter-experimenter reproducibility was less than 2%. The interchangeability of the two methods was studied by using the Bland-Altman method.

Conclusions: Together with PLS analysis, FT-IR spectrometry appears to be an easy-to-use and accurate method to determine multianalyte concentrations in dried human plasma. It could be an alternative tool for rapidly quantifying many molecules after developing a specific predictive model.

Clin Chem Lab Med 2009;47:83–90.

Keywords: immunoglobulins; multivariate analysis; plasma molecules; Fourier transform infrared (FT-IR) spectrometry; validation

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