Jump to ContentJump to Main Navigation
Show Summary Details

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Payne, Deborah A. / Schlattmann, Peter / Tate, Jillian R.

IMPACT FACTOR increased in 2015: 3.017
Rank 5 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2015: 0.873
Source Normalized Impact per Paper (SNIP) 2015: 0.982
Impact per Publication (IPP) 2015: 2.238

249,00 € / $374.00 / £187.00*

See all formats and pricing


Select Volume and Issue
Loading journal volume and issue information...

30,00 € / $42.00 / £23.00

Get Access to Full Text

Approaching clinical proteomics: current state and future fields of application in fluid proteomics

Rolf Apweiler1, b / Charalampos Aslanidis2 / Thomas Deufel3, a / Andreas Gerstner4, c / Jens Hansen2 / Dennis Hochstrasser5, d / Roland Kellner6, b / Markus Kubicek7, e / Friedrich Lottspeich8, b / Edmund Maser9 / Hans-Werner Mewes10 / Helmut E. Meyer11, b / Stefan Müllner12, b, f / Wolfgang Mutter13, b / Michael Neumaier14, a / Peter Nollau15, a / Hans G. Nothwang16, a / Fredrik Ponten17 / Andreas Radbruch18, c / Knut Reinert19 / Gregor Rothe20, a / Hannes Stockinger21, c, f / Attila Tarnok22 / Mike J. Taussig23 / Andreas Thiel18, c / Joachim Thiery24, a / Marius Ueffing25, b / Günther Valet8, c / Joel Vandekerckhove26 / Wiltrud Verhuven27 / Christoph Wagener15, a / Oswald Wagner7, e, f / Gerd Schmitz2, a, b, f

1European Bioinformatics Institute, Welcome Trust Genome Campus, Hinxton, UK

2Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Regensburg, Germany

3Institute for Clinical Chemistry and Laboratory Diagnostics, University of Jena, Jena, Germany

4Department of Otorhinolaryngology/Surgery, University of Bonn, Bonn, Germany

5Department of Pathology/Clinical Chemistry, University of Geneva, Geneva, Switzerland

6Merck, Darmstadt, Germany

7Institute of Medical and Chemical Laboratory Diagnostics, University of Vienna, Vienna, Austria

8Max-Planck-Institute for Biochemistry, Martinsried/Munich, Germany

9Institute of Toxicology and Pharmacology, University Kiel, Kiel, Germany

10Institute for Bioinformatics, GSF, Neuherberg, Germany

11Medical Proteomics Center, University of Bochum, Bochum, Germany

12Protagen, Dortmund, Germany

13PROFOS, Regensburg, Germany

14Institute for Clinical Chemistry, University Hospital Mannheim, Mannheim, Germany

15Institute for Clinical Chemistry, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

16Department of Animal Physiology, Technical University Kaiserslautern, Kaiserslautern, Germany

17Institute for Genetics and Pathology, University of Uppsala, Uppsala, Sweden

18German Rheumatism Research Center Berlin, Berlin, Germany

19Department of Mathematics and Computer Science, University of Berlin, Berlin, Germany

20Laboratory Center Bremen, Bremen, Germany

21Department of Molecular Immunology, University of Vienna, Vienna, Austria

22Department of Pediatric Cardiology, University of Leipzig, Leipzig, Germany

23Technology Research Group, The Babraham Institute Cambridge, Cambridge, UK

24Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University of Leipzig, Leipzig, Germany

25Institute of Human Genetics, GSF Neuherberg, Neuherberg, Germany

26Department of Medical Protein Research, University of Gent, Gent, Belgium

27Waters, Eschborn, Germany

aConsensus document of the DGKL – Deutsche Vereinte Gesellschaft für Klinische Chemie und Laboratorioumsmedizin (www.dgkl.de)

bDGPF – Deutsche Gesellschaft für Proteomforschung (www.dgpf.org)

cDGfZ – Deutsche Gesellschaft für Zytometrie (www.dgfz.org)

dSSCC – Schweizerische Gesellschaft für Klinische Chemie (www.sscc.ch)

eÖGLMKC – Österreichische Gesellschaft für Laboratoriumsmedizin und Klinische Chemie (www.oeglmkc.at)

fDanubian Biobank Consortium (www.danubianbiobank.de)

Corresponding author: Prof. Dr. med. Gerd Schmitz, Institute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, Franz-Josef-Strauß Allee 11, 93053 Regensburg, Germany Phone: +49-941-944-6201, Fax: +49-941-944-6202,

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 47, Issue 6, Pages 724–744, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2009.167, June 2009

Publication History



The field of clinical proteomics offers opportunities to identify new disease biomarkers in body fluids, cells and tissues. These biomarkers can be used in clinical applications for diagnosis, stratification of patients for specific treatment, or therapy monitoring. New protein array formats and improved spectrometry technologies have brought these analyses to a level with potential for use in clinical diagnostics. The nature of the human body fluid proteome with its large dynamic range of protein concentrations presents problems with quantitation. The extreme complexity of the proteome in body fluids presents enormous challenges and requires the establishment of standard operating procedures for handling of specimens, increasing sensitivity for detection and bioinformatical tools for distribution of proteomic data into the public domain. From studies of in vitro diagnostics, especially in clinical chemistry, it is evident that most errors occur in the preanalytical phase and during implementation of the diagnostic strategy. This is also true for clinical proteomics, and especially for fluid proteomics because of the multiple pretreatment processes. These processes include depletion of high-abundance proteins from plasma or enrichment processes for urine where biological variation or differences in proteolytic activities in the sample along with preanalytical variables such as inter- and intra-assay variability will likely influence the results of proteomics studies. However, before proteomic analysis can be introduced at a broader level into the clinical setting, standardization of the preanalytical phase including patient preparation, sample collection, sample preparation, sample storage, measurement and data analysis needs to be improved. In this review, we discuss the recent technological advances and applications that fulfil the criteria for clinical proteomics, with the focus on fluid proteomics. These advances relate to preanalytical factors, analytical standardization and quality-control measures required for effective implementation into routine laboratory testing in order to generate clinically useful information. With new disease biomarker candidates, it will be crucial to design and perform clinical studies that can identify novel diagnostic strategies based on these techniques, and to validate their impact on clinical decision-making.

Clin Chem Lab Med 2009;47:724–44.

Keywords: cerebrospinal fluid (CSF); clinical proteomics; fluid proteomics; mass spectrometry (MS); matrix assisted laser desorption/ionization (MALDI); preanalytical effects; standard operating procedures (SOP); surface-enhanced laser desorption/ionization (SELDI)

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Georgia Ikonomou, Martina Samiotaki, and George Panayotou
Critical Reviews in Clinical Laboratory Sciences, 2009, Volume 46, Number 5-6, Page 319
Simone Lista, Sid E. O’Bryant, Kaj Blennow, Bruno Dubois, Jacques Hugon, Henrik Zetterberg, Harald Hampel, and Yong Shen
Journal of Alzheimer's Disease, 2015, Volume 47, Number 2, Page 291
Rakesh Sharma, Harsha Gowda, Sandip Chavan, Jayshree Advani, Dhanashree Kelkar, G. S. Sameer Kumar, Mitali Bhattacharjee, Raghothama Chaerkady, T. S. Keshava Prasad, Akhilesh Pandey, Dindagur Nagaraja, and Rita Christopher
Journal of Proteome Research, 2015, Volume 14, Number 6, Page 2466
Anton B. Iliuk, Justine V. Arrington, and Weiguo Andy Tao
ELECTROPHORESIS, 2014, Volume 35, Number 24, Page 3430
Franck Bonnier, Matthew J. Baker, and Hugh J. Byrne
Analytical Methods, 2014, Volume 6, Number 14, Page 5155
Ming Guo, Ling He, P.J. Strong, and Hailong Wang
Chemosphere, 2014, Volume 112, Page 472
Wellington J. Rody Jr, L. Shannon Holliday, Kevin P. McHugh, Shannon M. Wallet, Victor Spicer, and Oleg Krokhin
American Journal of Orthodontics and Dentofacial Orthopedics, 2014, Volume 145, Number 6, Page 787
Colin T Dollery
British Journal of Pharmacology, 2014, Volume 171, Number 9, Page 2269
Yongjing Cheng, Yuling Chen, Xiaolin Sun, Yuhui Li, Cibo Huang, Haiteng Deng, and Zhanguo Li
Inflammation, 2014, Volume 37, Number 5, Page 1459
Kwang Hyun Ko, Chang Il Kwon, So Hye Park, Na Young Han, Hoo Keun Lee, Eun Hee Kim, and Ki Baik Hahm
Clinical Endoscopy, 2013, Volume 46, Number 6, Page 611
Harald Hampel, Simone Lista, Stefan J. Teipel, Francesco Garaci, Robert Nisticò, Kaj Blennow, Henrik Zetterberg, Lars Bertram, Charles Duyckaerts, Hovagim Bakardjian, Alexander Drzezga, Olivier Colliot, Stéphane Epelbaum, Karl Broich, Stéphane Lehéricy, Alexis Brice, Zaven S. Khachaturian, Paul S. Aisen, and Bruno Dubois
Biochemical Pharmacology, 2014, Volume 88, Number 4, Page 426
Franck Bonnier, François Petitjean, Matthew J. Baker, and Hugh J. Byrne
Journal of Biophotonics, 2014, Volume 7, Number 3-4, Page 167
Maciej Kaźmierczak, Magdalena Luczak, Krzysztof Lewandowski, Luiza Handschuh, Anna Czyż, Małgorzata Jarmuż, Michał Gniot, Michał Michalak, Marek Figlerowicz, and Mieczysław Komarnicki
Medical Oncology, 2013, Volume 30, Number 4
Eduardo Buzolin Barbosa, Alessandra Vidotto, Giovana Mussi Polachini, Tiago Henrique, Alessandra Bernadete Trovó de Marqui, and Eloiza Helena Tajara
Revista da Associação Médica Brasileira, 2012, Volume 58, Number 3, Page 366
Eduardo Buzolin Barbosa, Alessandra Vidotto, Giovana Mussi Polachini, Tiago Henrique, Alessandra Bernadete Trovó de Marqui, and Eloiza Helena Tajara
Revista da Associação Médica Brasileira (English Edition), 2012, Volume 58, Number 3, Page 366
Marie Grandjean, Marc Dieu, Martine Raes, and Olivier Feron
Journal of Immunological Methods, 2013, Volume 396, Number 1-2, Page 23
Eduardo Buzolin Barbosa, Alessandra Vidotto, Giovana Mussi Polachini, Tiago Henrique, Alessandra Bernadete Trovó de Marqui, and Eloiza Helena Tajara
Revista da Associação Médica Brasileira, 2012, Volume 58, Number 3, Page 366
J. Giner Martínez-Sierra, F. Moreno Sanz, P. Herrero Espílez, J. M. Marchante Gayón, J. Rodríguez Fernández, and J. I. García Alonso
Analytical and Bioanalytical Chemistry, 2013, Volume 405, Number 9, Page 2889
David L. Tabb
Clinical Biochemistry, 2013, Volume 46, Number 6, Page 411
Q.-Y. Hu, J. Su, H. Jiang, L.-L. Wang, and Y.-Q. Jia
International Journal of Laboratory Hematology, 2013, Volume 35, Number 4, Page 367
Ákos Végvári, Tadashi Kondo, and John G. Marshall
International Journal of Proteomics, 2012, Volume 2012, Page 1
Simone Lista, Frank Faltraco, David Prvulovic, and Harald Hampel
Progress in Neurobiology, 2013, Volume 101-102, Page 1
André C. Müller, Florian P. Breitwieser, Heinz Fischer, Christopher Schuster, Oliver Brandt, Jacques Colinge, Giulio Superti-Furga, Georg Stingl, Adelheid Elbe-Bürger, and Keiryn L. Bennett
Journal of Proteome Research, 2012, Volume 11, Number 7, Page 3715
SS Hasson, MS Al-Balushi, and AA Al-Jabri
Asian Pacific Journal of Tropical Disease, 2012, Volume 2, Number 2, Page 159
Stuart J. Cordwell, Alistair V. G. Edwards, Kiersten A. Liddy, Lia Moshkanbaryans, Nestor Solis, Benjamin L. Parker, Andy S. C. Yong, Clement Wong, Leonard Kritharides, Brett D. Hambly, and Melanie Y. White
Journal of Proteome Research, 2012, Volume 11, Number 4, Page 2114
Lorelei D. Shoemaker, Achal S. Achrol, Palaniappan Sethu, Gary K. Steinberg, and Steven D. Chang
Neurosurgery, 2012, Volume 70, Number 3, Page 518
Job D. Cardoza, Jignesh R. Parikh, Scott B. Ficarro, and Jarrod A. Marto
Wiley Interdisciplinary Reviews: Systems Biology and Medicine, 2012, Volume 4, Number 2, Page 141
Laura L. Elo and Benno Schwikowski
Wiley Interdisciplinary Reviews: Data Mining and Knowledge Discovery, 2012, Volume 2, Number 1, Page 1
Serena E. O'Neil, Bo Lundbäck, and Jan Lötvall
Journal of Proteomics, 2011, Volume 75, Number 1, Page 192
Irina A. Buhimschi and Catalin S. Buhimschi
Clinics in Perinatology, 2010, Volume 37, Number 2, Page 355
Pierre Lescuyer, Annarita Farina, and Denis F. Hochstrasser
Trends in Biotechnology, 2010, Volume 28, Number 5, Page 225
Allison Hubel, Alptekin Aksan, Amy P.N. Skubitz, Chris Wendt, and Xiao Zhong
Biopreservation and Biobanking, 2011, Volume 9, Number 3, Page 237
Pavel Gromov, Irina Gromova, Jakob Bunkenborg, Teresa Cabezon, José M.A. Moreira, Vera Timmermans-Wielenga, Peter Roepstorff, Fritz Rank, and Julio E. Celis
Molecular Oncology, 2010, Volume 4, Number 1, Page 65
Helen M. Moore, Andrea B. Kelly, Scott D. Jewell, Lisa M. McShane, Douglas P. Clark, Renata Greenspan, Daniel F. Hayes, Pierre Hainaut, Paula Kim, Elizabeth Mansfield, Olga Potapova, Peter Riegman, Yaffa Rubinstein, Edward Seijo, Stella Somiari, Peter Watson, Heinz-Ulrich Weier, Claire Zhu, and Jim Vaught
Journal of Proteome Research, 2011, Volume 10, Number 8, Page 3429
Helen M. Moore, Andrea Kelly, Scott D. Jewell, Lisa M. McShane, Douglas P. Clark, Renata Greenspan, Pierre Hainaut, Daniel F. Hayes, Paula Kim, Elizabeth Mansfield, Olga Potapova, Peter Riegman, Yaffa Rubinstein, Edward Seijo, Stella Somiari, Peter Watson, Heinz-Ulrich Weier, Claire Zhu, and Jim Vaught
Biopreservation and Biobanking, 2011, Volume 9, Number 1, Page 57
C Herder, M Karakas, and W Koenig
Clinical Pharmacology & Therapeutics, 2011, Volume 90, Number 1, Page 52
Annalisa Castagna, Oliviero Olivieri, Alberto Milli, Miriam Dal Bosco, Anna Maria Timperio, Lello Zolla, Muthukumar Gunasekaran, Ricciarda Raffaelli, Francesca Pizzolo, and Daniela Cecconi
PROTEOMICS - Clinical Applications, 2011, Volume 5, Number 5-6, Page 343
Helen M. Moore, Andrea B. Kelly, Scott D. Jewell, Lisa M. McShane, Douglas P. Clark, Renata Greenspan, Daniel F. Hayes, Pierre Hainaut, Paula Kim, Elizabeth A. Mansfield, Olga Potapova, Peter Riegman, Yaffa Rubinstein, Edward Seijo, Stella Somiari, Peter Watson, Heinz-Ulrich Weier, Claire Zhu, and Jim Vaught
Cancer Cytopathology, 2011, Volume 119, Number 2, Page 92
Jennifer W. Bea, Nicole C. Wright, Patricia Thompson, Chengcheng Hu, Stefano Guerra, and Zhao Chen
Clinical Chemistry and Laboratory Medicine, 2011, Volume 49, Number 5
Sam Hanash
OMICS: A Journal of Integrative Biology, 2011, Volume 15, Number 3, Page 133
Cinzia Magagnotti, Isabella Fermo, Rose Mary Carletti, Maurizio Ferrari, and Angela Bachi
Clinical Chemistry and Laboratory Medicine, 2010, Volume 48, Number 4
Kristina Schwamborn, Nadine T Gaisa, and Corinna Henkel
Expert Review of Proteomics, 2010, Volume 7, Number 6, Page 897
Amir Rahbar, Robert Rivers, Emily Boja, Christopher Kinsinger, Mehdi Mesri, Tara Hiltke, and Henry Rodriguez
Personalized Medicine, 2011, Volume 8, Number 1, Page 45
Ching-Seng Ang, Jason Phung, and Edouard C. Nice
Biomedical Chromatography, 2011, Volume 25, Number 1-2, Page 82
Ediri Sideso, Michalis Papadakis, Cynthia Wright, Ashok Handa, Alastair Buchan, Benedikt Kessler, and James Kennedy
Translational Stroke Research, 2010, Volume 1, Number 4, Page 304
Qin Fu, Florian S. Schoenhoff, William J. Savage, Pingbo Zhang, and Jennifer E. Van Eyk
PROTEOMICS - Clinical Applications, 2010, Volume 4, Number 3, Page 271
Raj Chari, Kelsie L. Thu, Ian M. Wilson, William W. Lockwood, Kim M. Lonergan, Bradley P. Coe, Chad A. Malloff, Adi F. Gazdar, Stephen Lam, Cathie Garnis, Calum E. MacAulay, Carlos E. Alvarez, and Wan L. Lam
Cancer and Metastasis Reviews, 2010, Volume 29, Number 1, Page 73
Muriel De Bock, Dominique de Seny, Marie-Alice Meuwis, Jean-Paul Chapelle, Edouard Louis, Michel Malaise, Marie-Paule Merville, and Marianne Fillet
Journal of Biomedicine and Biotechnology, 2010, Volume 2010, Page 1
Attila Tárnok
Cytometry Part A, 2009, Volume 75A, Number 10, Page 811

Comments (0)

Please log in or register to comment.