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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Editorial Board Member: Gillery, Philippe / Kazmierczak, Steven / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Whitfield, John B.

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Common reference intervals for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and γ-glutamyl transferase (GGT) in serum: results from an IFCC multicenter study

Ferruccio Ceriotti1 / Joseph Henny2 / Josep Queraltó3 / Shen Ziyu4 / Yeşim Özarda5 / Baorong Chen6 / James C. Boyd7 / Mauro Panteghini8 /

1Diagnostica e Ricerca S. Raffaele, Istituto Scientifico Universitario S. Raffaele, Milano, Italy

2Laboratoire de Biologie Clinique, Centre de Médicine Préventive, Vandoeuvre-lès-Nancy, France

3Servei de Bioquímica, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain

4National Center for Clinical Laboratory, Beijing Hospital Compound, Beijing, P.R. China

5Uludag University Medical School, Department of Biochemistry and Clinical Biochemistry, Bursa, Turkey

6Beijing Aerospace General Hospital, Beijing, P.R. China

7University of Virginia Health System, Department of Pathology, Charlottesville, VA, USA

8Centro Interdipartimentale per la Riferibilità Metrologica in Medicina di Laboratorio (CIRME), Università degli Studi, Milano, Italy

Corresponding author: Ferruccio Ceriotti, Diagnostica e Ricerca S. Raffaele, Via Olgettina 60, 20132 Milano, Italy Phone: +39 0226432282, Fax: +39 0226432640,

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 48, Issue 11, Pages 1593–1601, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2010.315, October 2010

Publication History

Published Online:
2010-10-30

Abstract

Background: Aspartate aminotransferase (AST), alanine aminotransferase (ALT) and γ-glutamyl transferase (GGT) measurements are important for the assessment of liver damage. The aim of this study was to define the reference intervals (RIs) for these enzymes in adults, paying attention to standardization of the methods used and careful selection of the reference population.

Methods: AST, ALT and GGT were measured with commercial analytical systems standardized to the IFCC-recommended reference measurement systems. Three centers (two in Italy and one in China) measured their own freshly collected samples; one of these centers also measured frozen samples from the Nordic Countries RI Project and from a Turkish center. RIs were generated using non-parametric techniques from the results of 765 individuals (411 females and 354 males, 18–85 years old) selected on the basis of the results of other laboratory tests and a specific questionnaire.

Results: AST results from the four regions (Milan, Beijing, Bursa and Nordic Countries) were statistically different, but these differences were too small to be clinically relevant. Likewise, differences between the upper reference limits for genders was only 1.7 U/L (0.03 μkat/L), allowing a single RI of 11–34 U/L (0.18–0.57 μkat/L) to be defined. Interregional differences were not statistically significant for ALT, but partitioning was required due to significant gender differences. RIs for ALT were 8–41 U/L (0.13–0.68 μkat/L) for females and 9–59 U/L (0.15–0.99 μkat/L) for males, respectively. The upper reference limits for GGT from the Nordic Country population were higher than those from the other three regions and results from this group were excluded from final calculations. The GGT RIs were 6–40 U/L (0.11–0.66 μkat/L) for females and 12–68 U/L (0.20– 1.13 μkat/L) for males, respectively.

Conclusions: For AST and ALT, the implementation of common RIs appears to be possible, because no differences between regions were observed. However, a common RI for GGT that is applicable worldwide appears unlikely due to differences among populations.

Clin Chem Lab Med 2010;48:1593–601.

Keywords: alanine aminotransferase; aspartate aminotransferase; γ-glutamyl transferase; reference intervals

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