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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Editorial Board Member: Gillery, Philippe / Kazmierczak, Steven / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Whitfield, John B.

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Age- and gender-dependent changes in circulating concentrations of tumor necrosis factor-α, soluble tumor necrosis factor receptor-1 and sulfated glycosaminoglycan in healthy people

1 / Pawel Olczyk1 / Katarzyna Winsz-Szczotka1 / Katarzyna Klimek2 / Krystyna Olczyk1

1Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia, Sosnowiec, Poland

2Department of Statistics, Medical University of Silesia, Sosnowiec, Poland

Corresponding author: Katarzyna Komosinska-Vassev, Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia, Jednosci 8, 41-200 Sosnowiec, Poland Phone: +48 32 3641150, Fax: +48 32 3641157

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 49, Issue 1, Pages 121–127, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2011.007, October 2010

Publication History

Received:
2010-04-23
Accepted:
2010-07-12
Published Online:
2010-10-20

Abstract

Background: In this study, the effect of gender and physio-logical ageing on circulating concentrations of plasma sulfated glycosaminoglycans (sGAG) as well as molecules involved in pro- (tumor necrosis factor-α; TNF-α) and anti-inflammatory responses (soluble tumor necrosis factor receptor-1, sTNF-RI) were assessed. The relationships between sGAG and molecules involved in age-dependent extracellular matrix (ECM) remodeling during physiological ageing were also investigated.

Methods: Circulating TNF-α and sTNF-RI were measured in 91 healthy volunteers using enzyme-linked immunosorbent assays. sGAG were quantified using an Alcian blue-binding assay.

Results: A linear age-related decline in plasma sGAG was found during the first five decades of life (r=–0.61, p<0.05), followed by an increase occurring only in females (r=0.46, p<0.05). Circulating TNF-α concentrations were inversely correlated with age (r=–0.24, p<0.05) over the lifetime. For TNF-α, the observed changes were gender specific. Serum sTNF-RI concentrations were not affected by age in either men or women. A significant positive correlation was found between the concentrations of TNF-α and both sGAG (r=0.22, p<0.05) and sTNF-RI (r=0.21, p<0.05).

Conclusions: Our data demonstrate that physiological ageing is associated with ECM remodeling, reflected by plasma sGAGs concentrations. Changes in the ECM metabolism during the ageing process were influenced by circulating TNF-α. Furthermore, serum concentrations of biomolecules involved in pro- and anti-inflammatory responses are not increased in healthy elderly subjects.

Keywords: extracellular matrix; glycosaminoglycans; physiological ageing; soluble tumor necrosis factor receptor; tumor necrosis factor-α

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