Clinical Chemistry and Laboratory Medicine (CCLM)
Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)
Editor-in-Chief: Plebani, Mario
Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R.
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Interlaboratory study of free monoclonal immunoglobulin light chain quantification
1Institute of Clinical Biochemistry and Diagnostics, Charles University Hospital, Hradec Kralove, Czech Republic
2Division of Clinical Haematology, 2nd Department of Medicine, Charles University Hospital, Hradec Kralove, Czech Republic
3Czech Myeloma Group, Czech Republic
4Department of Clinical Biochemistry and Haematology, Masaryk University Faculty of Medicine and University Hospital, Brno, Czech Republic
5Department of Allergy and Clinical Immunology, Kralovske Vinohrady University Hospital, Prague, Czech Republic
6Institute of Clinical Biochemistry and Haematology, Charles University Medical School and University Hospital, Pilsen, Czech Republic
7Department of Clinical Biochemistry, Palacky University Hospital, Olomouc, Czech Republic
8Institute of Clinical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University, Prague, Czech Republic
9Department of Oncology and 1st Faculty of Medicine, Thomayer University Hospital and Charles University, Prague, Czech Republic
10Babak Research Institute and Department of Internal Medicine and Hematooncology, Masaryk University Faculty of Medicine and University Hospital, Brno, Czech Republic
Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 49, Issue 1, Pages 89–92, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2011.019, October 2010
- Published Online:
Background: Quantification of monoclonal immunoglobulin free light chains (FLCs) in serum is used increasingly in clinical practice for the diagnosis, prognostic assessment, and treatment monitoring of monoclonal gammopathies. It is used as an adjunct to standard serum protein electrophoresis and immunofixation. However, methods for FLC quantification need further standardization and validation.
Methods: The Czech Myeloma Group and the Czech Society of Clinical Biochemistry have initiated an interlaboratory study where six laboratories collaborating with the primary myeloma treatment centres measured FLC concentrations in 12 serum samples from patients with monoclonal gammopathies.
Results: Repeatability of the measurements in five laboratories was calculated based on differences between the results of duplicate measurements. We found that repeatability depended more on the laboratory than on the device used for measurement.
Conclusions: The study revealed several weak points in the methodology, including the need for a uniform sample dilution procedure. Interlaboratory reproducibility was comparable with values achieved in the NEQAS programme. Because the κ/λ ratio cannot be measured with high precision, κ and λ FLC concentrations should be used where possible. Due to its impact on the clinical management of patients with gammopathy, FLC quantification needs to become a part of the regular quality control cycle in myeloma centres.
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