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Licensed Unlicensed Requires Authentication Published by De Gruyter February 11, 2011

Identification of Bcl-2/IgH fusion sequences using real-time PCR and chip-based microcapillary electrophoresis

  • Yoko Tabe EMAIL logo , Yukiko Kawase , Kazunori Miyake , Naotake Satoh , Nanae Aritaka , Yasushi Isobe , Kazuo Oshimi , Norio Komatsu , Takashi Miida and Akimichi Ohsaka

Abstract

Background: The determination of polymerase chain reaction (PCR) amplification product sizes of the Bcl-2/IgH fusion gene from follicular lymphoma (FL) provides evidence of clonal identity.

Methods: The present study describes detection of Bcl-2/IgH fusion gene clonality utilizing a small, simple microcapillary electrophoretic chip combined with a real-time PCR method.

Results: The microcapillary electrophoretic chip system effectively detects size differences among the Bcl-2/IgH fusion gene amplification products of FL from patient samples; something that is not possible using traditional gel electrophoresis. We also describe the potential of this system to utilize formalin-fixed, paraffin-embedded tissue samples sectioned on charged slides.

Conclusions: The simple detection of Bcl-2/IgH fusion gene clonality using a microcapillary electrophoretic chip provides reliable information for monitoring minimal residual disease of FL, and can be an effective tool for use in clinical laboratories.


Corresponding author: Yoko Tabe, MD, PhD, Department of Clinical Laboratory Medicine, Juntendo University School of Medicine, Hongo 2-1-1, Bunkyo-ku, Tokyo 113-8421, Japan Phone: +81-3-3813-3111 (ex) 5187, Fax: +81-3-5804-8637

Received: 2010-9-5
Accepted: 2010-12-15
Published Online: 2011-02-11
Published in Print: 2011-05-01

©2011 by Walter de Gruyter Berlin New York

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