Jump to ContentJump to Main Navigation

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.

12 Issues per year


IMPACT FACTOR 2014: 2.707
Rank 6 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2014: 0.741
Source Normalized Impact per Paper (SNIP) 2014: 1.011
Impact per Publication (IPP) 2014: 2.310

VolumeIssuePage

Issues

The antinuclear antibody assay: developing criteria for reflexive anti-dsDNA antibody testing in a laboratory setting

1 / Thomas B. Ledue1

1Foundation for Blood Research, Scarborough, ME, USA

Corresponding author: Wendy Y. Craig, Foundation for Blood Research, P.O. Box 190, Scarborough, ME 04070-0190, USA Phone: +1-207-883-4131, Fax: +1-207-883-1377

Citation Information: Clinical Chemistry and Laboratory Medicine. Volume 49, Issue 7, Pages 1205–1211, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/CCLM.2011.613, May 2011

Publication History

Received:
2011-01-17
Accepted:
2011-03-01
Published Online:
2011-05-26

Abstract

Background: We examined the relationship between antinuclear antibody (ANA) data and the presence of anti-double stranded DNA antibodies (anti-dsDNA).

Methods: De-identified demographic, ANA and anti-dsDNA data were available for 30,196 individuals aged ≥20 years, whose sera were submitted sequentially to our laboratory. When multiple sera were received for the same subject, data from the earliest sample were used. Anti-dsDNA frequency was stratified by ANA titer and pattern, sample referral source, and by the patient's age, gender, and diagnosis.

Results: For sera with ANA titer ≥256 and an accompanying diagnosis of systemic lupus erythematosus, anti-dsDNA frequency was 53.7%, 35.3%, and 37.5% for homogeneous, speckled, or multiple ANA patterns, respectively. Among remaining sera with ANA titer ≥256, anti-dsDNA frequency was highest for the homogeneous pattern (15.9%). Anti-dsDNA frequency was three-fold higher among sera submitted by rheumatologists compared with other providers. However, its relative distribution by ANA pattern and titer was similar between these groups. Patient age and gender had no significant effect on anti-dsDNA frequency after ANA data were taken into account.

Conclusions: ANA pattern and titer, together with the diagnosis submitted with the serum sample, can be used to guide decisions for reflexive anti-dsDNA testing in a clinical laboratory setting.

Keywords: anti-dsDNA antibody; antinuclear antibody; indirect immunofluorescence assay

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Vijay Rao and Caroline Gordon
Immunotherapy, 2014, Volume 6, Number 11, Page 1165
[2]
Anupama Tiwari and Upendra Kumar Soni
Medical Hypotheses, 2014, Volume 83, Number 6, Page 825
[3]
George K. Bertsias, Cristina Pamfil, Antonios Fanouriakis, and Dimitrios T. Boumpas
Nature Reviews Rheumatology, 2013, Volume 9, Number 11, Page 687
[4]
Wendy Y. Craig and Thomas B. Ledue
Clinical Chemistry and Laboratory Medicine, 2012, Volume 50, Number 3

Comments (0)

Please log in or register to comment.