Jump to ContentJump to Main Navigation

Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.

13 Issues per year

IMPACT FACTOR 2014: 2.707
Rank 6 out of 30 in category Medical Laboratory Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2014: 0.741
Source Normalized Impact per Paper (SNIP) 2014: 1.011
Impact per Publication (IPP) 2014: 2.310



Evaluation of Maxwell® 16 for automated DNA extraction from whole blood and formalin-fixed paraffin embedded (FFPE) tissue

1 / Midori Mitui1 / Nora Kristine Leos1 / Beverly Barton Rogers2, 3 / Jason Y. Park1, 4

1Department of Pathology, Children’s Medical Center, Dallas, TX, USA

2Department of Pathology, Children’s Healthcare of Atlanta, Atlanta, GA, USA

3Emory University, Atlanta, GA, USA

4Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA

Corresponding author: Shama Khan Khokhar, Department of Pathology, Children’s Medical Center, 1935 Medical District Dr, Dallas, TX 75235, USA Phone: +1-214-456-7253

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM). Volume 50, Issue 2, Pages 267–272, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/cclm.2011.763, October 2011

Publication History

Published Online:


Background: The aim of the study was to assess the performance of Promega, Maxwell® 16 for the extraction of genomic DNA from whole blood and FFPE tissue.

Methods: DNA was extracted from 10 whole blood and 10 FFPE specimens using six different commercial kits.

Results: For whole blood, the mean DNA concentration obtained by Maxwell® 16 was significantly greater than either easyMAG® (p<0.0001) or QIAamp® Blood DNA kit (p<0.001). For FFPE, the mean DNA concentration obtained by the AllPrep® FFPE specific DNA/RNA kit was significantly greater than either the Maxwell® 16 (p<0.0001) or the general AllPrep® DNA/RNA kit (p<0.0001).

Conclusions: Comparative evaluation of the six DNA extraction kits indicated that the semi-automated Maxwell® 16 was superior for whole blood extraction while the manual AllPrep® FFPE DNA/RNA kit (Qiagen) performed better for FFPE DNA extraction in terms of quantity of DNA obtained. All six extraction methods (blood and FFPE) performed well in terms of purity. Although there were variances in the quantity of DNA obtained, there were no significant differences in the efficiency of these methods in yielding amplifiable DNA extracts, as demonstrated by β-actin for whole blood specimens. In evaluation of FFPE DNA extraction methods, the Qiagen AllPrep® FFPE DNA/RNA Mini Kit was the best for applications requiring larger amplicons, but for smaller amplicons the Maxwell was most consistent.

Keywords: automation; method validation; nucleic acid extraction

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Rafael Gutiérrez-López, Josué Martínez-de la Puente, Laura Gangoso, Ramón C. Soriguer, and Jordi Figuerola
Journal of Vector Ecology, 2015, Volume 40, Number 1, Page 11
Tae-Dong Jeong, Young-Uk Cho, Woochang Lee, Sail Chun, and Won-Ki Min
Clinica Chimica Acta, 2014, Volume 435, Page 14
Sean X Zhang
Future Microbiology, 2013, Volume 8, Number 12, Page 1599
A. Bernhardt, L. de Boni, H.A. Kretzschmar, and K. Tintelnot
Der Pathologe, 2013, Volume 34, Number 6, Page 540
Serena Bonin and Giorgio Stanta
Expert Review of Molecular Diagnostics, 2013, Volume 13, Number 3, Page 271
Patrick Guertler, Andrea Harwardt, Adelina Eichelinger, Paul Muschler, Ottmar Goerlich, and Ulrich Busch
European Food Research and Technology, 2013, Volume 236, Number 4, Page 599

Comments (0)

Please log in or register to comment.