Lupus anticoagulant testing: analyzing fresh samples after a single centrifugation and after a 6–8 h delay : Clinical Chemistry and Laboratory Medicine (CCLM)

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Clinical Chemistry and Laboratory Medicine (CCLM)

Published in Association with the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM)

Editor-in-Chief: Plebani, Mario

Ed. by Gillery, Philippe / Lackner, Karl J. / Lippi, Giuseppe / Melichar, Bohuslav / Schlattmann, Peter / Tate, Jillian R. / Tsongalis, Gregory J.


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Lupus anticoagulant testing: analyzing fresh samples after a single centrifugation and after a 6–8 h delay

1 / Mira Barak1

1Regional Laboratory, Haifa and the Western Galilee, Clalit Health Services, Nesher, Israel

Corresponding author: Professor Paul Froom, Hematology Department, Central Laboratory of Haifa and the Western Galilee, Clalit Health Services, 20200 Nesher, Israel Phone: +972-506261353, Fax: +972-4-8209029

Citation Information: Clinical Chemistry and Laboratory Medicine (CCLM). Volume 50, Issue 2, Pages 367–370, ISSN (Online) 1437-4331, ISSN (Print) 1434-6621, DOI: 10.1515/cclm.2011.768, October 2011

Publication History

Received:
2011-09-20
Accepted:
2011-10-03
Published Online:
2011-10-31

Abstract

Background: For automation it is important to know the effect of analyzing fresh samples after a single rather than after two centrifugations, and to determine test stability.

Methods: This study compared silica clotting times (SCT) and dilute Russel’s viper venom tests (dRVVT) after one and two centrifugations in 50 fresh plasma samples. Then it compared test results within 4 h to those after 6–8 h of blood drawing in 40 samples. Means, minimums, maximums and quartiles of the paired screen were compared, test ratios were confirmed and correlations, linear regressions and Bland-Altman statistics were calculated.

Results: The distributions of test results were nearly identical, regardless of the number of centrifugations or timing of the analysis. The first centrifugation explained 97.7% and 94.8% of the variance of tests results after the second centrifugation for the SCT and dRVVT, respectively. The test results after 6–8 h explained 98.3% and 96.3% of the variance of the SCT-ratios and dRVVT-ratios, respectively, tested within 4 h. Inter-day coefficients of correlation of ratio comparisons were similar to those of the controls values.

Conclusions: Testing of fresh samples after a single centrifugation might replace batch testing of frozen samples after double centrifugation, providing timelier reporting of results and resulting in savings of technician time.

Keywords: centrifugation; dilute Russel’s viper venom test; fresh plasma; lupus anticoagulant; platelets; silica clotting test; stability

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