International Journal of the Biology, Chemistry, Physics, and Technology of Wood
Editor-in-Chief: Faix, Oskar
Editorial Board Member: Daniel, Geoffrey / Militz, Holger / Rosenau, Thomas / Salmen, Lennart / Sixta, Herbert / Vuorinen, Tapani / Argyropoulos, Dimitris S. / Balakshin, Yu / Barnett, J. R. / Berry, Richard / Burgert, Ingo / Evans, Robert / Evtuguin, Dmitry V. / Frazier, Charles E. / Fukushima, Kazuhiko / Gellerstedt, Göran / Gindl-Altmutter, Wolfgang / Glasser, W. G. / Heitner, Cyril / Holmbom, Bjarne / Isogai, Akira / Kadla, John F. / Kleen, Marjatta / Koch, Gerald / Lachenal, Dominique / Mansfield, Shawn D. / Morrell, J.J. / Niemz, Peter / Pizzi, Antonio / Ragauskas, Arthur J. / Ralph, John / Rice, Robert W. / Salin, Jarl-Gunnar / Schmitt, Uwe / Schultz, Tor P. / Sipilä, Jussi / Tamminen, Tarja / Viikari, Liisa / Welling, Johannes / Willför, Stefan / Yoshihara, Hiroshi
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Impact per Publication (IPP) 2015: 1.427
Application of Scanning UV Microspectrophotometry to Localise Lignins and Phenolic Extractives in Plant Cell Walls
Citation Information: Holzforschung. Volume 55, Issue 6, Pages 563–567, ISSN (Print) 0018-3830, DOI: 10.1515/HF.2001.091, June 2005
- Published Online:
The localisation of lignin and phenolic extractives in woody tissue was determined using scanning UV microspectrophotometry. This improved cellular analytical technique enabled direct imaging of the topochemical lignin distribution within individual cell wall layers with a resolution of 0.25 μm2. Selected softwood (Picea abies), hardwood (Fagus sylvatica, Entandrophragma cylindricum, Prunus serotina) and monocotyledon (Phyllostachys edulis) sections of 1 μm thickness were scanned at a fixed wavelength and evaluated with the “APAMOS” software. This approach allowed the distribution pattern of lignins and aromatic extractives within the cell wall to be visualised simultaneously. The method was found to be ideally suited to the study of their subcellular distribution in plant cell walls.
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