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Modeling of Phytase Production by Cultivation of Pichia pastoris Under the Control of the GAP Promoter
1University of Ottawa, email@example.com
2University of Ottawa, firstname.lastname@example.org
3University of Ottawa, email@example.com
4University of Ottawa, firstname.lastname@example.org
Citation Information: International Journal of Chemical Reactor Engineering. Volume 8, Issue 1, Pages –, ISSN (Online) 1542-6580, DOI: 10.2202/1542-6580.2144, January 2010
- Published Online:
Cell growth and recombinant phytase production under the control of the GAP promoter were studied in continuous cultivation experiments of Pichia pastoris. Based on these studies, a simple kinetic model was established to predict biomass and enzyme production. Although parameters were mainly estimated using the results of continuous cultivation, the proposed model was able to successfully predict cell growth and phytase production in three different fed-batch cultivations of P. pastoris using limited glucose feeding.