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International Journal of Occupational Medicine and Environmental Health

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IL-18 Levels in Nasal Lavage After Inhalatory Challenge Test with Flour in Bakers Diagnosed with Occupational Asthma

Anna Krakowiak1 / Jolanta Walusiak1 / Patrycja Krawczyk1 / Marta Wiszniewska1 / Wojciech Dudek1 / Tomasz Wittczak1 / Cezary Pałczyński1,

Department of Occupational Diseases, Nofer Institute of Occupational Medicine, Łódź, Poland1

Centre of Occupational and Environmental Allergy, Nofer Institute of Occupational Medicine, Łódź, Poland2

This content is open access.

Citation Information: International Journal of Occupational Medicine and Environmental Health. Volume 21, Issue 2, Pages 165–172, ISSN (Online) 1896-494X, ISSN (Print) 1232-1087, DOI: 10.2478/v10001-008-0015-y, July 2008

Publication History

Published Online:
2008-07-09

IL-18 Levels in Nasal Lavage After Inhalatory Challenge Test with Flour in Bakers Diagnosed with Occupational Asthma

Objectives: The authors discuss the outcomes of a study on IL-18 concentration in nasal washings after the inhalatory challenge test with flour allergens (ICHT-F) in bakers with flour-induced occupational airway allergy (OAA) Methods: We measured IL-18 concentration using ELISA kit and assessed morphological changes in nasal lavage fluid (NLF) before, and 4 h and 24 h after ICHT-F in three groups of subjects: Group A — 9 patients with diagnosed OAA (occupational asthma and rhinitis), Group B — 10 patients with atopic asthma and rhinitis, and Group C — 9 healthy volunteers. Results: In Group A, significant differences in the basophil proportion in NLF were noted only 24 h after ICHT-F. Both the basophil proportion and total eosinophil count were higher in Group A than in Group C at this time-point. Group A also showed a statistically significant increase in IL-18 levels 4 h after the challenge. A significant relationship was noted between the proportion of basophils 4 h after ICHT-F and IL-18 level at 24 h after the test. Conclusions: This is the first study demonstrating an increased expression of IL-18 in nasal washings of subjects diagnosed with OAA to flour allergens. The observed higher concentrations of IL-18 in nasal washings after ICHT as well as the increase in the proportion of basophils provide evidence for the important role of IL-18 in persistent allergic inflammation.

Keywords: IL-18; Diagnostics; Occupational asthma

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