It is well documented that homophobia exists among healthcare providers including nurses. However, little research is available on the level of homophobia among nursing students and nursing faculty. Using the Index of Attitudes Toward Homosexuals (IAH) and the Homophobic Behavior of Students Scale (HBSS) 241 nursing students and 32 faculty in a Midwest university were invited to participate. One hundred twenty six students completed the survey resulting in a 51% return rate. Fifteen faculty completed the survey. Results reveal that there is a low level of homophobia among students and faculty at this university. Religion and LGBT acquaintances or family members accounted for most of the variance within the homophobia scores. While scores reflect low levels of homophobia, it is the belief of these researchers that they actually may reflect ambivalent or heterosexist attitudes toward LGBT people which may impact healthcare delivered by these future nurses.