University students in this millennium will be older, culturally diverse and likely to change their career major several times during the course of their education. Methods to identify and guide appropriate students toward nursing are lacking. The purpose of this study was to develop and initially test a 48-item Likert-scaled instrument, the Career Search Questionnaire (CSQ), which assesses interest in and self-efficacy for nursing. Data were obtained from 300 volunteer students, with implied consent, enrolled in beginning-level courses at a Midwestern university. Response differences were apparent between individuals who reported nursing as a college major compared to those who sought a different major. Participants who reported nursing as their major obtained higher scores in both interest (p = 0.00) and self-efficacy (p = 0.00). The CSQ is psychometrically sound, with a reliability score of 0.87 and capable of identifying the student for whom nursing may be the suitable career.