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Interdisciplinary Toxicology

The Journal of Institute of Experimental Pharmacology of Slovak Academy of Sciences


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Investigation of in vitro effects of ethephon and chlorpyrifos, either alone or in combination, on rat intestinal muscle contraction

Mustafa Çetinkaya1 / Emine Baydan1

National Food Reference Laboratory, Turkish Ministry of Agriculture and Rural Affairs, Fatih Sultan Mehmet Bulvari, Yenimahalle, Ankara, Turkey1

Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ankara, Diskapi, Ankara, Turkey2

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Citation Information: Interdisciplinary Toxicology. Volume 3, Issue 1, Pages 35–39, ISSN (Online) 1337-9569, ISSN (Print) 1337-6853, DOI: 10.2478/v10102-010-0002-6, April 2010

Publication History

Published Online:
2010-04-13

Investigation of in vitro effects of ethephon and chlorpyrifos, either alone or in combination, on rat intestinal muscle contraction

A range of pesticides is widely used in pest management and the chances of exposure to multiple organophosphorus (OP) compounds simultaneously are high, especially from dietary and other sources. Although health hazards of individual OP insecticides have been relatively well characterized, there is lesser information on the interactive toxicity of multiple OP insecticides. The aim of this study is to elicit the possible interactions in case combined exposure of an OP pesticide chlorpyrifos (CPF) and a plant growth regulator ethephon (ETF) which are used worldwide. The ileum segments of 3 months old Wistar Albino male rats were used in isolated organ bath containing Tyrode solution. ETF and CPF were incubated (10-7 M concentration) separately or in combination with each other to ileum and their effects on acetylcholine-induced contractions were studied. The data obtained from this study show that, single and combined exposure to the agents caused agonistic interactions with regard to potency of ACh whereas they caused a decrease on Emax value of ACh. These findings suggest that exposure to these agents which have direct and indirect cholinergic effects, may cause developing clinical responses with small doses and earlier but the extent of toxicity will be lower.

Keywords: ethephon; chlorpyrifos; acetylcholine; combination; organophosphate

  • Axelrad JC, Howard CV and McLean WG. (2002). Interactions between pesticides and components of pesticide formulations in an in vitro neurotoxicity test. Toxicology 173: 259-268.

  • Becerra MA, Herrera MD and Marhuenda E. (2001). Action of tacrine on muscarinic receptors in rat intestinal smooth muscle. J Auton Pharmacol 21: 113-119. [PubMed]

  • Chanda SM, Mortensen SR, Moser VC, Padilla S. (1997). Tissue-Spesific Effects of Chlorpyrifos on Carboxylesterase and Cholinesterase Activity in Adult Rats: An in Vitro and in Vivo Comparison. Fundam App Toxicol 38: 148-157. [CrossRef]

  • Cochran RC. (2002). Appraisal of Risks from Nonoccupational Exposure to Chlorpyrifos. Reg Toxicol Pharmacol 35: 105-121. [CrossRef]

  • Cook TJ and Shenoy SS. (2003). Intestinal permeability of chlorpyrifos using the single-pass intestinal perfusion method in the rat. Toxicology 184: 125-133.

  • El-Masri HA, Mumtaz MM and Yushak ML. (2004). Application of physiologically-based pharmacokinetic modeling to investigate the toxicological interaction between chlorpyrifos and parathion in the rat. Environ Tox Pharm 16: 57-71.

  • EPA. (1995). Reregistration Eligibility Decision (RED): Ethephon. List A Case 0382. U.S. Environmental Protection Agency. Office of Prevention, Pesticides and Toxic Substances. Washington, D.C. 20460. Available from: http://www.epa.gov/REDs/0382.pdf

  • Haux JE, Lockridge O and Casida JE. (2002). Specificity of Ethephon as a Butyrylcholinesterase Inhibitor and Phosphorylating Agent. Chem Res Toxicol 15: 1527-1533. [PubMed] [CrossRef]

  • Haux JE, Quistad GB and Casida JE. (2000). Phosphobutyrylcholinesterase: Phosphorylation of the Esteratic Site of Butyrylcholinesterase by Ethephon [(2-Chloroethyl)phosphonic Acid] Dianion. Chem Res Toxicol 13: 646-651. [CrossRef] [PubMed]

  • Howard MD and Pope CN. (2002). In vitro effects of chlorpyrifos, parathion, methyl parathion and their oxons on cardiac muscarinic receptor binding in neonatal and adult rats. Toxicology 170: 1-10.

  • Karanth S, Liu J, Olivier KJr, Pope C. (2004). Interactive toxicity of the organophosphorus insecticides chlorpyrifos and methyl parathion in adult rats. Toxicol Appl Pharmacol 196: 183-190.

  • Kousba AA, Poet TS and Timchalk C. (2003). Characterization of the in vitro kinetic interaction of chlorpyrifos-oxon with rat salivary cholinesterase: A potential biomonitoring matrix. Toxicology 188: 219-232.

  • Poet TS, Wu H, Kousba AA, Timchalk C. (2003). In Vitro Rat Hepatic and Intestinal Metabolism of the Organophosphate Pesticides Chlorpyrifos and Diazinon. Toxicol Sci 72: 193-200. [PubMed] [CrossRef]

  • Pope C, Karanth S and Liu J. (2005). Pharmacology and toxicology of cholinesterase inhibitors: uses and misuses of a common mechanism of action. Environ Tox Pharm 19: 433-446. [CrossRef]

  • Segall Y, Grendell RL, Toia RF, Casida JE. (1991). Composition of Technical Ethephon [(2-Chloroethyl)phosphonic Acid] and Some Analogues Relative to Their Reactivity and Biological Activity. J Agric Food Chem 39: 380-385. [CrossRef]

  • Smegal DC. (2000). Human Health Risk Assessment: Chlorpyrifos. U.S. Environmental Protection Agency. Office of Pesticide Programs, Health Effects Division (7509C). Available from: http://www.epa.gov/pesticides/op/chlorpyrifos/hedrra.pdf

  • Sparks SE, Quistad GB and Casida JE. (1999). Organophosphorus Pesticide-Induced Butyrylcholinesterase Inhibition and Potentiation of Succinylcholine Toxicity in Mice. J Biochem Molecular Tox 13: 113-118. [CrossRef]

  • Taha MO, Fraga MM, Fagundes DJ, Bandeira CP, Caricati-Neto A, Jurkiewicz A. (2002). Effect of Preservation Conditions on Autonomic Transmission in Rat Small Bowel. Transplantation Proceedings 34: 1021-1024. [CrossRef] [PubMed]

  • Tezuka A, Ishihata A, Aita T, Katano Y. (2004). Aging-related alterations in the contractile responses to acetylcholine, muscarinic cholinoceptors and cholinesterase activities in jejunum and colon of the male Fischer 344 rats. Experimental Gerontology 39: 91-100. [CrossRef]

  • Timchalk C, Nolan RJ, Mendrala AL, Dittenber DA, Brzak KA, Mattsson JL. (2002). A physiologically based pharmacokinetic and pharmacodynamic (PBPK/PD) model for the organophosphate insecticide chlorpyrifos in rats and humans. Toxicol Sci 66: 34-53. [CrossRef] [PubMed]

  • Timchalk C, Poet TS, Hinman MN, Busby AL, Kousba AA. (2005). Pharmacokinetic and pharmacodynamic interaction for a binary mixture of chlorpyrifos and diazinon in the rat. Toxicol Appl Pharmacol 205: 31-42.

  • Tomlin CDS. (1997). The Pesticide Manual: A World Compendium. 11th Ed. The British Crop Protection Council.

  • Zhang N and Casida JE. (2002). Novel Irreversible Butyrylcholinesterase Inhibitors: 2-Chloro-1-(substituted-phenyl)ethylphosphonic Acids. Bioorg Med Chem 10: 1281-1290.

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