Hygrophila spinosa T. Anderson, commonly known as Gokulakanta (Hygrophila) is a well known medicinal plant found in all over India. The different parts of the plant are used to treat various diseases in the folklore medicine as the seeds are given by hakims with “sugar, milk or wine” for the treatment of impotence, gonorrhea and spermatorrhoea. Active constituents such as steroids and glycosides which are under investigation, reported to possess spermatogenetic potential and the phytochemical screening has shown positive for steroids and glycosides. The present study have been carried out to investigate and evaluate the aphrodisiac activity and spermatogenetic potential of ethanolic extract of the seeds of H. spinosa (EEHS) on ethanol induced rat model for testicular injury. The EEHS at two dose levels 100 and 200 mg/kg b.w. administered orally for 60 days to the ethanol induced rats produced significant (P <0.01) reversal of biochemical change in serum (Total protein, cholesterol and testosterone level) and testicular intoxication and also shows significant (P <0.01) spermatogenic activity by increase in fertility. Both doses showed significant aphrodisiac activity by increase in mounting behavior and mating performance. From the observation, it could be predicted that aphrodisiac and spermatogenic effect of EEHS against infertility may be due to hormonal and neurohumoral changes, which play an important role in the sexual behavior and fertility disorders.