Mitragyna parvifolia (Roxb.) Korth (Rubiaceae) is widely used as an acrid, anodyne, bitter, stomachic, febrifuge, and in the treatment of gastropathy, colic, fever, and inflammation. In the present study, the anti-inflammatory and antinociceptive properties of the stem bark methanol extract (MPBM) extracted by Soxhlet extraction and alkaloid rich fraction (MPBAlk) of M. parvifolia was investigated on various animal models. MPBM extract and MPBAlk fraction when tested separately, showed statistically significant (P < 0.01) anti-inflammatory activity by inhibition of percentage rise in paw edema as compared to the control group in a dose dependent manner at different time intervals. When evaluated in acetic acid?induced writings, hot plate and tail flick method in mice, MPBM extract and MPBAlk fraction showed significant analgesic activity at higher dose of 400 mg/kg, whereas activity was moderate at lower dose. These findings suggest that M. parvifolia stem-bark possess significant and dose dependent anti-inflammatory and antinociceptive effects. Chemical analysis shows the presence of alkaloids, flavonoids, glycosides and tannins which may be responsible for these activities.