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Measurement Science Review

The Journal of Institute of Measurement Science of Slovak Academy of Sciences

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Nonlinear Optical Measurements of the Artery Wall: Parameters Related to the Progression of Atherosclerosis

Michael Sowa1 / Leila Mostaco-Guidolin1 / Michael Smith1 / Elica Kohlenberg1 / Andrew Ridsdale1 / Albert Stolow1 / Alex Ko1

Institute for Biodiagnostics, National Research Council Canada, Winnipeg, Canada, R3B 1Y61

Steacie Institute for Molecular Sciences, National Research Council Canada, Ottawa, Canada, K1A 0R62

This content is open access.

Citation Information: Measurement Science Review. Volume 9, Issue 4, Pages 93–94, ISSN (Online) 1335-8871, ISSN (Print) , DOI: 10.2478/v10048-009-0018-2, September 2009

Publication History

Published Online:
2009-09-03

Nonlinear Optical Measurements of the Artery Wall: Parameters Related to the Progression of Atherosclerosis

Nonlinear optical (NLO) microscopy is used to follow key structural and biochemical changes associated with the progression of atherosclerosis. Arteries from WHHL-MI rabbits are examined using a 3 channel NLO microscope that can simultaneously monitor the coherent anti-stokes Raman scattered light (CARS), the two-photon excited fluorescence (TPEF) and the second harmonic generation (SHG) from a sample. Distinct differences in the nonlinear optical signals are observed that correlate with the age of the vessel and the presence of atherosclerotic plaque. These differences are attributed to the changing extracellular matrix and the increased lipid deposition associated with plaque development. The capability of NLO to perform 3D sectioning in thick highly scattering vessels in order to visualize structural details of the artery wall and highlight vessel pathology is demonstrated. These features make NLO a potentially valuable tool to help understand the progression of atherosclerosis.

Keywords: Nonlinear optical microscopy; coherent anti-Stokes Raman scattering; two photon excited fluorescence; second harmonic generation; atherosclerosis

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