Jump to ContentJump to Main Navigation

Polish Journal of Veterinary Sciences

The Journal of Committee of Veterinary Sciences of Polish Academy of Sciences and University of Warmia and Mazury in Olsztyn

4 Issues per year

IMPACT FACTOR increased in 2013: 0.712

Open Access
VolumeIssuePage

Open Access

Induction of immune responses in sheep by vaccination with liposome-entrapped DNA complexes encoding Toxoplasma gondii MIC3 gene

E. Hiszczyńska-Sawicka1, / H. Li1 / J. Xu1 / M. Akhtar1 / L. Holec-Gąsior1 / J. Kur1 / R. Bickerstaffe1 / M. Stankiewicz1

Faculty of Agriculture and Life Sciences, Lincoln University, PO Box 84, Lincoln, Canterbury, New Zealand1

Immunoparasitology Laboratory, Department of Parasitology, University of Agriculture, Faisalabad, Pakistan2

Department of Microbiology, Chemical Faculty, Gdańsk University of Technology, Narutowicza 11/12, 80-952 Gdańsk, Poland3

This content is open access.

Citation Information: Polish Journal of Veterinary Sciences. Volume 15, Issue 1, Pages 3–9, ISSN (Print) 1505-1773, DOI: 10.2478/v10181-011-0107-7, April 2012

Publication History

Published Online:
2012-04-06

Induction of immune responses in sheep by vaccination with liposome-entrapped DNA complexes encoding Toxoplasma gondii MIC3 gene

Toxoplasma gondii is a parasite that has been extensively studied due to its medical and veterinary importance in terminating pregnancies. Consequently, a satisfactory vaccine is required to control its adverse effects on pregnant animals. The microneme protein, MIC3, is a major adhesion protein that binds to the surface of host cells and parasites, and is therefore a potential vaccine against T. gondii. The viability of MIC3 as a vaccine is investigated in this study. Sheep were injected twice, intramuscularly, with plasmids containing DNA encoding for the mature form of MIC3 protein formulated into liposomes. Control sheep were injected with an empty vector or received no injections. The injection of sheep with DNA plasmids encoding for MIC3 elicited an immune response after the first and second injections as indicated by antibody responses and the production of IFN-γ. The immune response, as measured by the IgG2 and IgG1 serum levels, was boosted after the injection of the MIC3 DNA vaccine together with high anti-MIC3 antibodies.

The results demonstrate that the intramuscular injection of sheep with a plasmid containing DNA coding for MIC3 protein induces a significant and effective immune response against T. gondii.

Keywords: DNA vaccine; Toxoplasma gondii; MIC3 antigen

  • Beghetto E, Nielsen HV, Del Porto P, Buffolano W, Guglietta S, Felici F, Petersen E, Gargano N (2005) A combination of antigenic regions of Toxoplasma gondii microneme proteins induces protective immunity against oral infection with parasite cysts. J Infect Dis 191: 637-645.

  • Beláková J, Horynová M, Krupka M, Weigl E, Raska M (2007) DNA vaccines: are they still just a powerful tool for the future? Arch Immunol Ther Exp (Warsz) 55: 387-398. [Web of Science]

  • Buxton D, Maley SW, Wright SE, Rodger S, Bartley P, Innes EA (2007) Toxoplasma gondii and ovine toxoplasmosis: new aspects of an old story. Vet Parasitol 149: 25-28. [Web of Science]

  • Cerede O, Dubremetz JF, Bout D, Lebrun M (2002) The Toxoplasma gondii protein MIC3 requires pro-peptide cleavage and dimerization to function as adhesin. EMBO J 21: 2526-2536.

  • Chardès T, Bourguin I, Mévélec MN, Dubremetz JF, Bout D (1990) Antibody responses to Toxoplasma gondii in sera, intestinal secretions and milk from orally infected mice and characterization of target antigens. Infect Immun 58: 1240-1246.

  • Dautu G, Munyaka B, Carmen G, Zhang G, Omata Y, Xuenan X, Igarashi M (2007) Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential. Exp Parasitol 116: 273-282. [Web of Science]

  • Gazzinelli RT, Amichay D, Sharton-Kersten T, Grunwald E, Farber JM, Sher A (1996) Role of macrophage-derived cytokines in the induction and regulation of cell-mediated immunity to Toxoplasma gondii. Curr Top Microbiol Immunol 219: 127-139.

  • Hiszczyńska-Sawicka E, Brillowska-Dąbrowska A, Dąbrowski S, Pietkiewicz H, Myjak P, Kur J (2003) High yield expression and single-step purification of Toxoplasma gondii SAG1, GRA1 and GRA7 antigens in Escherichia coli. Prot Expr Purif 27: 150-157.

  • Hiszczyńska-Sawicka E, Kur J, Pietkiewicz H, Holec L, Gąsior A, Myjak P (2005) Efficient production of the Toxoplasma gondii GRA6, p35 and SAG2 recombinant antigens and their applications in the serodiagnosis of toxoplasmosis. Acta Parasitol 50: 249-254.

  • Hiszczyńska-Sawicka E, Li H, Xu JB, Olędzka G, Kur J, Bickerstaffe R, Stankiewicz M (2010a) Comparison of immune response in sheep immunized with DNA vaccine encoding Toxoplasma gondii GRA7 antigen in different adjuvant formulations. Exp Parasitol 124: 365-372. [Web of Science]

  • Hiszczyńska-Sawicka E, Akhtar M, Kay GW, Holec-Gasior L, Bickerstaffe R, Kur J, Stankiewicz M (2010b) The immune responses of sheep after DNA immunization with Toxoplasma gondii MAG1 antigen - with and without co-expression of ovine interleukin 6. Vet Immunol Immunopathol 136: 324-329. [Web of Science]

  • Holec-Gąsior L, Kur J, Hiszczyńska-Sawicka E (2009) GRA2 and ROP1 recombinant antigens as potential markers for detection of Toxoplasma gondii - specific immunoglobulin G in humans with acute toxoplasmosis. Clin Vaccine Immunol 16: 510-514. [Web of Science]

  • Ismael AB, Sekkai D, Collin C, Bout D, Mevelec MN (2003) The MIC3 gene of Toxoplasma gondii is a novel potent vaccine candidate against toxoplasmosis. Infect Immun 71: 6222-6228.

  • Ismael AB, Dimier-Poisson I, Lebrun M, Dubremetz JF, Bout D, Mevelec MN (2006) Mic1-3 knockout of Toxoplasma gondii is a successful vaccine against chronic and congenital toxoplasmosis in mice. J Infect Dis 194: 1176-1183.

  • Johnson LL, Sayles PC (2002) Deficient humoral responses underlie susceptibility to Toxoplasma gondii in CD-4 deficient mice. Infect Immun 70: 185-191.

  • Kang H, Remington JS, Suzuki Y (2000) Decreased resistance of B cell-deficient mice to infection with Toxoplasma gondii despite unimpaired expression of IFN-γ, TNF-α, and inducible nitric oxide synthase. J Immunol 164: 2629-2634.

  • Kur J, Holec-Gąsior L, Hiszczyńska-Sawicka E (2009) Current status of toxoplasmosis vaccine development. Expert Rev Vaccines 8: 791-808. [Web of Science]

  • McLeod R, Mack DG (1986) Secretory IgA specific for Toxoplasma gondii. J Immunol 136: 2640-2643.

  • Mévélec MN, Ducournau C, Bassuny Ismael A, Olivier M, Sèche E, Lebrun M, Bout D, Dimier-Poisson I (2010) Mic1-3 Knockout Toxoplasma gondii is a good candidate for a vaccine against T. gondii-induced abortion in sheep. Vet Res 41: 49.

  • Payne RW (2009) The Guide to GenStat® Release 12, Part 2: Statistics. VSN International, Hemel Hempstead.

  • Pietkiewicz H, Hiszczyńska-Sawicka E, Kur J, Petersen E, Nielsen HV, Stankiewicz M, Andrzejewska I, Myjak P (2004) Usefulness of Toxoplasma gondii-recombinant antigens in serodiagnosis of human toxoplasmosis. J Clin Microbiol 42: 1779-1781.

  • Pontarollo RA, Rankin R, Babiuk LA, Godson DL, Griebel PJ, Hecker R, Krieg AM, van Drunen Littel-van den Hurk S (2002) Monocytes are required for optimum in vitro stimulation of bovine peripheral blood mononuclear cells by non-methylated CpG motifs. Vet Immunol Immunopathol 84: 43-59.

  • Qu D, Yu H, Wang S, Cai W, Du A (2009) Induction of protective immunity by multiantigenic DNA vaccine delivered in attenuated Salmonella typhimurium against Toxoplasma gondii infection in mice. Vet Parasitol 166: 220-227.

  • Soldati D, Dubremetz JF, Lebrun M (2001) Microneme proteins: structural and functional requirements to promote adhesion and invasion by the apicomplexan parasite Toxoplasma gondii. Int J Parasitol 31: 1293-1302.

  • Suzuki Y, Orellana MA, Schreiber RD, Remington JS (1988) Interferon-gamma: the major mediator of resistance against Toxoplasma gondii. Science 240: 516-518.

  • Tomley FM, Soldati DS (2001) Mix and match modules: structure and function of microneme proteins in apicomplexan parasites. Trends Parasitol 17: 81-88.

  • Wood PR, Jones SL (2001) BOVIGAM: an in vitro cellular diagnostic test for bovine tuberculosis. Tuberculosis (Edinb) 81: 147-155.

  • Xiang W, Qiong Z, Li-Peng L, Kui T, Jian-Wu G, Heng-Ping S (2009) The location of invasion-related protein MIC3 of Toxoplasma gondii and protective effect of its DNA vaccine in mice Vet Parasitol 166: 1-7.

Comments (0)

Please log in or register to comment.