Jump to ContentJump to Main Navigation

In print for more than 50 years!

Radiochimica Acta

International Journal for chemical aspects of nuclear science and technology

Ed. by Qaim, Syed M.

12 Issues per year

IMPACT FACTOR 2014: 1.014
5-year IMPACT FACTOR: 1.226
Rank 16 out of 34 in category Nuclear Science & Technology in the 2014 Thomson Reuters Journal Citation Report/Science Edition

SCImago Journal Rank (SJR) 2014: 0.449
Source Normalized Impact per Paper (SNIP) 2014: 0.782
Impact per Publication (IPP) 2014: 1.187



Labelling and optimization of PHOTOFRIN® with 99mTc

M. Fakhar-e-Alam1 / Samina Roohi2 / M. Atif* / S. Firdous3 / Nagina Amir4 / R. Zahoor5

1 Pakistan Institute of Engineering and Applied Sciences

2 Pakistan Institute of Nuclear Science and Technology, Isotope Production Division, P.O. Nilore, Islamabad, Pakistan

3 National Institute of Laser and Optronics, Biophotonics Laboratory, Islamabad 45650, Pakistan

4 Isotope Production Division, Pakistan Insititute of Nuclear Science and Technol, Islamabad, Pakistan

5 Pakistan Institute of Nuclear Science and Technology, Isotope Production Division, Islamabad 45650, Pakistan

* Correspondence address: Pakistan Institute of Engineering and Applied Sciences, P.O.Nilore, Islamabad 45650, Pakistan,

Citation Information: Radiochimica Acta International journal for chemical aspects of nuclear science and technology. Volume 98, Issue 12, Pages 813–818, ISSN (Print) 0033-8230, DOI: 10.1524/ract.2010.1779, November 2010

Publication History

Published Online:


PHOTOFRIN® was labelled with 99mTc using SnCl2·2H2O as reducing agent. Instant thin layer chromatography (ITLC-SG) in 0.05 M NaOH was used for evaluation of radiochemical purity. Labelling efficiency was dependent on various factors that include the ligand/reductant ratio, pH and time of incubation. Therefore, optimum conditions of labelling were also determined. The stability of 99mTc-PHOTOFRIN® in serum was checked by using fresh human serum. Tissue distribution of 99mTc-PHOTOFRIN® was evaluated in Sprague Dawley rats.

PHOTOFRIN® was labelled with an efficiency of >95% under optimum conditions, which were PHOTOFRIN®: 200 μg, pH: 3–4, SnCl2·2H2O: 15 μg and 30 min incubation at room temperature. The 99mTc-labelled PHOTOFRIN® remained stable in human serum for 24 h. Biodistribution study in rats revealed maximum concentration of the labelled compound in liver, lungs and spleen at 0.5 h, and significant activity was also seen in the bladder and urine, indicating the mode of urinary excretion of PHOTOFRIN®.

Keywords: 99mTc-PHOTOFRIN®; Hematoporphyrin derivative; Quality control; Paper electrophoresis; Biodistribution

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

Joseph Sherma and Dolcie DeGrandchamp
Journal of Liquid Chromatography & Related Technologies, 2015, Volume 38, Number 3, Page 381
Pedro M. Santos, Mafalda Laranjo, Arménio C. Serra, Ana Margarida Abrantes, Marta Piñeiro, João Casalta-Lopes, Diná Trindade, Jorge Maia, António Rocha-Gonsalves, and Maria Filomena Botelho
Journal of Labelled Compounds and Radiopharmaceuticals, 2014, Volume 57, Number 3, Page 141
Jamshed Hussain Zaidi, Mohammad Wasim, Mohammad Arif, and A. Mushtaq
Radiochimica Acta, 2012, Volume 100, Number 8-9, Page 555
Muhammad Fakhar-e-Alam, Syed Muhammad Usman Ali, Zafar Hussain Ibupoto, Khun Kimleang, M. Atif, Muhammad Kashif, Foo Kai Loong, Uda Hashim, and Magnus Willander
Lasers in Medical Science, 2012, Volume 27, Number 3, Page 607
A. Khurshid, S. Firdous, L. Ahmat, J. Ferraria, J. D. Vollet-Filho, C. Kurachi, V. S. Bagneto, M. Nawaz, M. Ikram, and M. Ahmad
Laser Physics, 2012, Volume 22, Number 1, Page 317
M. Fakhar-e-Alam, S. M. U. Ali, Z. H. Ibupoto, M. Atif, and M. Willander
Laser Physics, 2011, Volume 21, Number 12, Page 2165
M. Atif, M. Fakhar-e-Alam, and M. S. AlSalhi
Laser Physics, 2011, Volume 21, Number 11, Page 1950
M. Fakhar-e-Alam, M. Atif, T. Rehman, H. Sadia, and S. Firdous
Laser Physics, 2011, Volume 21, Number 8, Page 1428
M. Atif, M. Fakhar-e-Alam, S. S. Z. Zaidi, and R. Suleman
Laser Physics, 2011, Volume 21, Number 6, Page 1135
M. Atif, M. Fakhar-e-Alam, L.G. Sabino, M. Ikram, M.T. de Araujo, C. Kurachi, V.S. Bagnato, and M.S. AlSalhi
Laser Physics Letters, 2011, Volume 8, Number 5, Page 386

Comments (0)

Please log in or register to comment.