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Radiology and Oncology

The Journal of Association of Radiology and Oncology

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The antitumor efficiency of combined electrochemotherapy and single dose irradiation on a breast cancer tumor model

Elham Raeisi1, / Seyed Aghamiri1 / Azin Bandi1 / Negar Rahmatpour1 / Seyed Firoozabadi1 / Sedigheh Kafi-Abad1 / Lluis Mir1, ,

Department of Medical Physics, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran1

Faculty of Science, University of Geneve, Geneve, Switzerland2

Division of Radiation Medicine, Shahid Beheshti University, Tehran, Iran3

Department of Immunohematology, Research Center of Iranian Blood Transfusion Organization (IBTO), Tehran, Iran4

CNRS, Orsay, Laboratoire de Vectorologie et Therapeutiques Anticancereuses, UMR 8203, 91405 Orsay cedex, France5

Laboratoire de Vectorologie et Therapeutiques Anticancereuses, Universite Paris-Sud, UMR 8203, 91405 Orsay cedex, France6

Institute Gustave Roussy, Laboratoire de Vectorologie et Therapeutiques Anticancereuses, UMR 8203, 114 rue E. Vaillant, 94805 Villejuif cedex, France7

This content is open access.

Citation Information: Radiology and Oncology. Volume 46, Issue 3, Pages 226–232, ISSN (Online) 1581-3207, ISSN (Print) 1318-2099, DOI: 10.2478/v10019-012-0035-x, June 2012

Publication History

Published Online:

The antitumor efficiency of combined electrochemotherapy and single dose irradiation on a breast cancer tumor model

Background. The aim of this study was to investigate the antitumor effectiveness of electrochemotherapy with cis-platin combined with suboptimal radiotherapy doses. Tumor radiosensitization was evaluated on large invasive ductal carcinoma tumors in Balb/C mice.

Materials and methods. Tumors of an average volume of 630 mm3 were treated with cisplatin, electric pulses, radiotherapy, electrochemotherapy, alone as well as in appropriate combinations. Tumors were irradiated with Cobalt-60 γ-rays at doses 3 Gy and 5 Gy in combination with electrochemotherapy using cisplatin. Controls included each of the treatments alone as well as the combination of the radiotherapy with electric pulses alone or with cisplatin alone. Antitumor effectiveness was evaluated by tumor growth delay, tumor-doubling time, inhibition ratio and the objective response rates.

Results. As anticipated, electrochemotherapy was more effective than the treatment with cisplatin alone or the application of the electric pulses alone. When treatments were combined with tumor irradiation at either 3 or 5 Gy, the combination with electrochemotherapy was more effective: at 5 Gy, 2 animals out of 8 were in complete remission 100 days later. In general the higher 5 Gy dose of γ-radiation was more effective than the lower one of 3 Gy.

Conclusions. The results of our study demonstrate that irradiation doses, 3 Gy or 5 Gy, increase the antitumor effectiveness of electrochemotherapy with cisplatin on invasive ductal carcinoma tumors. Good antitumor results were achieved in experimental tumors with a size comparable to clinical lesions, demonstrating that this three-modality combined treatment is useful for the treatment of large lesions even at sub-optimal radiotherapy doses.

Keywords: cisplatin; irradiation; electroporation; invasive ductal carcinoma; multimodalities

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