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Reviews in Analytical Chemistry

Editor-in-Chief: Schechter, Israel

Editorial Board Member: Pauw, Edwin / Vries, Mattanjah / Grushka, Eli / Laserna, J. / Licht, Stuart / Lubman, David / Mandler, Daniel / Palleschi, Vincenzo / Sigman, Michael / Whitesides, George / Yarnitzki, Chaim

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Analysis of clenbuterol residues in pig liver using liquid chromatography electrospray ionization tandem mass spectrometry

1 / Igor Bratoš2 / Ana Vulić1 / Nina Perši1 / Jelena Ðugum3

1Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska 143, 10 000 Zagreb, Croatia

2Pliva Research Institute, Prilaz Baruna Filipovića 29, 10 000 Zagreb, Croatia

3Ministry of Agriculture, Fisheries and Rural Development, Grada Vukovara 78, 10 000 Zagreb, Croatia

Citation Information: Reviews in Analytical Chemistry. Volume 30, Issue 1, Pages 5–9, ISSN (Online) 2191-0189, ISSN (Print) 0793-0135, DOI: 10.1515/revac.2011.008, April 2011

Publication History

Published Online:
2011-04-12

Abstract

The liquid chromatography-electrospray ionization tandem mass spectrometry method was developed for determination of clenbuterol residues in liver tissue as a regulatory matrix for the control of clenbuterol abuse as an anabolic. To investigate the level of clenbuterol residues during the withdrawal period, male food-producing pigs were exposed to subchronic repeat oral administration of a clenbuterol growth-promoting dose for 28 days. The analytical procedure showed acceptable validation results for all liver spiked samples analyzed and proved to be useful as a quantification and confirmation method in supporting regulatory enforcement programs of clenbuterol misuse monitoring. The highest level of clenbuterol in the liver of treated animals was recorded on day 0 of treatment cessation (21.58±14.29 ng/g), followed by 6.59±3.11 ng/g on day 3, 0.83±0.27 ng/g on day 7, and 0.44±0.08 ng/g on day 14 of withdrawal. At the end of the study period (day 35), the concentration of clenbuterol was below the method limit of detection (<0.1 ng/g).

Keywords: clenbuterol residues; liquid chromatography-electrospray ionization tandem mass spectrometry; liver; pigs

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