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Micellar-Enhanced Spectrofluorimetric Method for Quantification of Diclofenac Potassium in Pure Form, in Pharmaceutical Preparations and Human Plasma

Mit Mizellen unterstützte spektrofluorimetrische Methode zur Quantifizierung von Diclofenac-Kaliumsalz in reiner Form, in pharmazeutischen Zubereitungen und in menschlichem Plasma
  • Muhammad Naeem Khan

    Dr. Muhammad Naeem Khan received his PhD in Analytical Chemistry in 2013 from the Institute of Chemical Sciences University of Peshawar under the supervision of Prof. Dr. Muhammad Rasul Jan (Professor Emeritus) and Professor Dr. Jasmin Shah (Meritorious Professor). Dr. Muhamamd Naeem Khan is currently working as Assistant Professor of Chemistry in the Department of Chemistry Bacha Khan University Charsadda. Dr. Khan is supervising a number of MPhil, MSc and BS research students. He has published 22 papers in international journals.

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    , Irum

    Ms Irum has completed her MSc in analytical Chemistry. She did research in her MSc under the supervision of Dr. Muhammad Naeem Khan. Currently she is doing her MPhil under the supervision of Dr. Muhammad Naeem Khan. This manuscript is written by her under the guidance of Dr. Khan. Ms. Irum has passed all the courses with good grade.

    Ms Irum has completed her MSc in analytical Chemistry. She did research in her MSc under the supervision of Dr. Muhammad Naeem Khan. Currently she is doing her MPhil under the supervision of Dr. Muhammad Naeem Khan. This manuscript is written by her under the guidance of Dr. Khan. Ms. Irum has passed all the courses with good grade.

    , Saba Gul

    Ms Saba Gul has completed her MSc in analytical Chemistry. She did research in her MSc under the supervision of Dr. Muhammad Naeem Khan and this manuscript is based on her research work which she carried out along with her classmate Muslima. Ms. Saba Gul has passed all the courses with good grades.

    , Muslima

    Ms Muslima has completed her MSc in analytical Chemistry. She did research in MSc under the supervision of Dr. Muhammad Naeem Khan and this manuscript is from her research work which she carried out along with her classmate Saba Gul. Ms. Muslima has passed all the courses with good grades

    and Muhammad Mursaleen

    Mr. Muhammad Mursaleen has completed his BS in Analytical Chemistry and did research in BS under the supervision of Dr. Muhammad Naeem Khan. Currently he is enrolled in MPhil and doing research on method development and validation for pharmaceutical drugs. He has passed his MPhil courses with high grades and his research work is in progress.

Abstract

A rapid, simple and economical spectrofluorimetric method for the determination of diclofenac potassium in pure form, in pharmaceutical preparations and in human plasma has been developed. The method is based on the enhancement of the fluorescence signal of diclofenac potassium by the addition of sodium dodecyl sulphate in McIvaine buffer with a pH of 5. Different experimental conditions such as buffer type, pH, type and concentration of surfactants were investigated. The fluorescence intensity of the solution was recorded at 361 nm after excitation at 243 nm. The method shows linearity in the concentration range of 0.2 μg mL–1–10 μg mL–1 with a good correlation coefficient of 0.997. The relative standard deviation value was 3.62 (n = 7). The limit of detection and limit of quantification were calculated to be 2.84 × 10–3 μg mL–1 and 9.47 × 10–3 μg mL-1, respectively. The effect of excipients and co-administrated drugs was investigated and no interference was observed. The method was successfully applied for the determination of diclofenac potassium in pure form, in pharmaceutical products and in human plasma. The percentage recoveries obtained ranged from 100.25% to 102.16% for pure form and 97.50% to 102.00% for pharmaceutical products and from 98.50% to 101.67% for human plasma.

Zusammenfassung

Es wurde ein schnelles, einfaches und wirtschaftliches spektrofluorimetrisches Verfahren zur Bestimmung von Diclofenac-Kaliumalz in reiner Form, in pharmazeutischen Produkten und in menschlichem Plasma entwickelt. Die Methode basiert auf der Verstärkung des Fluoreszenzsignals von Diclofenac-Kaliumsalz durch die Zugabe von Natriumdodecylsulfat in McIvaine-Puffer mit einem pH-Wert von 5. Es wurden verschiedene Versuchsbedingungen wie Pufferart, pH-Wert, Art und Konzentration der Tenside untersucht. Die Fluoreszenzintensität der Lösung wurde bei 361 nm und nach Anregung bei 243 nm aufgezeichnet. Die Methode zeigt lineares Verhalten im Konzentrationsbereich von 0,2 μg mL–1–10 μg mL–1 mit einem guten Korrelationskoeffizienten von 0,997. Die relative Standardabweichung betrug 3,62 (n = 7). Die berechnete Nachweis- und Bestimmungsgrenze betrug 2,84 × 10–3 μg mL–1 bzw. 9,47 × 10–3 μg mL–1. Die Einfluss von Hilfsstoffen und gleichzeitig verabreichten Arzneimitteln wurde untersucht und es wurden keine Interferenzen festgestellt. Die Methode wurde erfolgreich für die Bestimmung von Diclofenac-Kalium in reiner Form, in pharmazeutischen Produkten und in menschlichem Plasma eingesetzt. Die erzielten prozentualen Wiederfindungen lagen zwischen 100,25% und 102,16% für die reine Form und zwischen 97,50% und 102,00% für pharmazeutische Produkte sowie zwischen 98,50% und 101,67% für Humanplasma.


Muhammad Naeem Khan Department of Chemistry Bacha Khan University 24420 Charsadda Pakistan

About the authors

Dr. Muhammad Naeem Khan

Dr. Muhammad Naeem Khan received his PhD in Analytical Chemistry in 2013 from the Institute of Chemical Sciences University of Peshawar under the supervision of Prof. Dr. Muhammad Rasul Jan (Professor Emeritus) and Professor Dr. Jasmin Shah (Meritorious Professor). Dr. Muhamamd Naeem Khan is currently working as Assistant Professor of Chemistry in the Department of Chemistry Bacha Khan University Charsadda. Dr. Khan is supervising a number of MPhil, MSc and BS research students. He has published 22 papers in international journals.

Ms Irum

Ms Irum has completed her MSc in analytical Chemistry. She did research in her MSc under the supervision of Dr. Muhammad Naeem Khan. Currently she is doing her MPhil under the supervision of Dr. Muhammad Naeem Khan. This manuscript is written by her under the guidance of Dr. Khan. Ms. Irum has passed all the courses with good grade.

Ms Irum has completed her MSc in analytical Chemistry. She did research in her MSc under the supervision of Dr. Muhammad Naeem Khan. Currently she is doing her MPhil under the supervision of Dr. Muhammad Naeem Khan. This manuscript is written by her under the guidance of Dr. Khan. Ms. Irum has passed all the courses with good grade.

Ms Saba Gul

Ms Saba Gul has completed her MSc in analytical Chemistry. She did research in her MSc under the supervision of Dr. Muhammad Naeem Khan and this manuscript is based on her research work which she carried out along with her classmate Muslima. Ms. Saba Gul has passed all the courses with good grades.

Ms Muslima

Ms Muslima has completed her MSc in analytical Chemistry. She did research in MSc under the supervision of Dr. Muhammad Naeem Khan and this manuscript is from her research work which she carried out along with her classmate Saba Gul. Ms. Muslima has passed all the courses with good grades

Mr. Muhammad Mursaleen

Mr. Muhammad Mursaleen has completed his BS in Analytical Chemistry and did research in BS under the supervision of Dr. Muhammad Naeem Khan. Currently he is enrolled in MPhil and doing research on method development and validation for pharmaceutical drugs. He has passed his MPhil courses with high grades and his research work is in progress.

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Received: 2020-07-05
Accepted: 2021-06-16
Published Online: 2021-11-30

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