Skip to content
BY-NC-ND 3.0 license Open Access Published by De Gruyter Open Access February 21, 2014

DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea

  • Lenka Hlavatá EMAIL logo , Vlastimil Vyskočil , Katarína Beníková , Monika Borbélyová and Ján Labuda
From the journal Open Chemistry

Abstract

Novel electrochemical DNA-based biosensors with outer-sphere Nafion and chitosan protective membranes were prepared for the evaluation of antioxidant properties of beverages (beer, coffee, and black tea) against prooxidant hydroxyl radicals. A carbon working electrode of a screen-printed three-electrode assembly was modified using a layer-by-layer deposition technique with low molecular weight double-stranded DNA and a Nafion or chitosan film. The membrane-covered DNA biosensors were initially tested with respect to their voltammetric and impedimetric response after the incubation of the beverage and the medium exchange for the solution of the redox indicator [Fe(CN)6]3−/4−. While the Nafion-protected biosensor proved to be suitable for beer and black tea extracts, the chitosan-protected biosensor was successfully used in a coffee extract. Afterwards, the applicability was successfully verified for these biosensors for the detection of a deep degradation of the surface-attached DNA at the incubation in the cleavage agent (hydroxyl radicals generated via Fenton reaction) and for the evaluation of antioxidant properties of coffee and black tea extracts against prooxidant hydroxyl radicals. The investigation of the novel biosensors with a protective membrane represents a significant contribution to the field of electrochemical DNA biosensors utilization.

[1] I. Leceta, P. Guerrero, K. de la Caba, Carbohydr. Polym. 93, 339 (2013) http://dx.doi.org/10.1016/j.carbpol.2012.04.03110.1016/j.carbpol.2012.04.031Search in Google Scholar

[2] M. Rinaudo, Prog. Polym. Sci. 31, 603 (2006) http://dx.doi.org/10.1016/j.progpolymsci.2006.06.00110.1016/j.progpolymsci.2006.06.001Search in Google Scholar

[3] C. K.S. Pillai, W. Paul, C.P. Sharma, Prog. Polym. Sci. 34, 641 (2009) http://dx.doi.org/10.1016/j.progpolymsci.2009.04.00110.1016/j.progpolymsci.2009.04.001Search in Google Scholar

[4] E. S. de Alvarenga, In: M. Elnashar (Ed.), Biotechnology of Biopolymers (InTech, Rijeka, 2011) 91 Search in Google Scholar

[5] M. N.V.R. Kumar, React. Funct. Polym. 46, 1 (2000) http://dx.doi.org/10.1016/S1381-5148(00)00038-910.1016/S1381-5148(00)00038-9Search in Google Scholar

[6] L. Yang, G.C. Wang, Y.J. Liu, J.J. An, M. Wang, Anal. Bioanal. Chem. 8, 2545 (2013) http://dx.doi.org/10.1007/s00216-012-6634-y10.1007/s00216-012-6634-ySearch in Google Scholar

[7] A. Mulyasuryani, A. Roosdiana, A. Srihardyastutie, Indo. J. Chem. 10, 162 (2010) 10.22146/ijc.21454Search in Google Scholar

[8] J. Galandova, J. Labuda, Chem. Papers 63, 1 (2009) http://dx.doi.org/10.2478/s11696-008-0083-210.2478/s11696-008-0083-2Search in Google Scholar

[9] M. Casciola, D. Capitani, A. Donnadio, V. Frittella, M. Pica, M. Sganappa, Fuel Cells 4, 381 (2009) http://dx.doi.org/10.1002/fuce.20080012810.1002/fuce.200800128Search in Google Scholar

[10] S. Poyrda, N. Jaffrezic-Renault, C. Martelet, S. Cosnier, P. Labble, Anal. Chim. Acta 364, 165 (1998) http://dx.doi.org/10.1016/S0003-2670(98)00184-610.1016/S0003-2670(98)00184-6Search in Google Scholar

[11] N. Foray, D. Marot, A. Gabriel, V. Randrianarison, A.M. Carr, M. Perricaudet, A. Ashworth, P. Jeggo, EMBO J. 22, 2860 (2003) http://dx.doi.org/10.1093/emboj/cdg27410.1093/emboj/cdg274Search in Google Scholar PubMed PubMed Central

[12] A. Barzilai, G. Rotman, Y. Shiloh, DNA Repair 1, 3 (2002) 10.1016/S1568-7864(01)00007-6Search in Google Scholar

[13] M. Valko, D. Leibfritz, J. Moncol, M. Cronin, M. Mazur, J. Telser, Int. J. Biochem. Cell Biol. 39, 44 (2007) http://dx.doi.org/10.1016/j.biocel.2006.07.00110.1016/j.biocel.2006.07.001Search in Google Scholar

[14] M. Fojta, In: E. Palecek, F. Scheller, J. Wang (Eds.), Electrochemistry of Nucleic Acids and Proteins — Towards Electrochemical Sensors for Genomics and Proteomics (Elsevier, Amsterdam, 2005) 385 10.1016/S1871-0069(05)01012-8Search in Google Scholar

[15] J. Labuda, In: M. Mascini, I. Palchetti (Eds.), Nucleic Acid Biosensors for Environmental Pollution Monitoring (Royal Society of Chemistry, Cambridge, 2011) 121 Search in Google Scholar

[16] J. Labuda, V. Vyskocil, in: K. Ota, G. Kreysa, R.F. Savinell (Eds.), Encyclopedia of Applied Electrochemistry (Springer, Berlin, 2014) “DNA/ Electrode Interface — Detection of Damage to DNA Using DNA-Modified Electrodes” 10.1007/978-1-4419-6996-5_259Search in Google Scholar

[17] G. Ziyatdinova, J. Labuda, Anal. Methods 3, 2777 (2011) http://dx.doi.org/10.1039/c1ay05403a10.1039/c1ay05403aSearch in Google Scholar

[18] L. D. Mello, L.T. Kubota, Talanta 72, 335 (2007) http://dx.doi.org/10.1016/j.talanta.2006.11.04110.1016/j.talanta.2006.11.041Search in Google Scholar PubMed

[19] R. Apak, S. Gorinstein, V. Bohm, K.M. Schaich, M. Ozyurek, K. Guclu, Pure Appl. Chem. 85, 957 (2013) http://dx.doi.org/10.1351/PAC-REP-12-07-1510.1351/PAC-REP-12-07-15Search in Google Scholar

[20] N. Pellegrini, M. Serafini, B. Colombi, D. Del Rio, S. Salvatore, M. Bianchi, F. Brighenti, J. Nutr. 133, 2812 (2003) 10.1093/jn/133.9.2812Search in Google Scholar PubMed

[21] J. Sochor, J. Dobes, O. Krystofova, B. Ruttkay-Nedecky, P. Babula, M. Pohanka, T. Jurikova, O. Zitka, V. Adam, B. Klejdus, R. Kizek, Int. J. Electrochem. Sci. 8, 8464 (2013) Search in Google Scholar

[22] G. Ziyatdinova, I. Aytuganova, A. Nizamova, H. Budnikov, Food Anal. Methods 6, 1629 (2013) http://dx.doi.org/10.1007/s12161-013-9591-y10.1007/s12161-013-9591-ySearch in Google Scholar

[23] G. K. Ziyatdinova, A.M. Nizamova, I.I. Aytuganova, H.C. Budnikov, J. Anal. Chem 68, 132 (2013) http://dx.doi.org/10.1134/S106193481302017210.1134/S1061934813020172Search in Google Scholar

[24] I. Novak, M. Seruga, S. Komorsky-Lovric, Food Chem. 122, 1283 (2010) http://dx.doi.org/10.1016/j.foodchem.2010.03.08410.1016/j.foodchem.2010.03.084Search in Google Scholar

[25] J. Labuda, M. Buckova, L. Heilerova, S. Silhar, I. Stepanek, Anal. Bioanal. Chem. 376, 168 (2003) 10.1007/s00216-003-1884-3Search in Google Scholar PubMed

[26] L. Heilerova, M. Buckova, P. Tarapcik, S. Silhar, J. Labuda, Czech. J. Food Chem. 21, 78 (2003) 10.17221/3480-CJFSSearch in Google Scholar

[27] G. Ziyatdinova, J. Labuda, Electroanalysis 24, 2333 (2012) http://dx.doi.org/10.1002/elan.20120041610.1002/elan.201200416Search in Google Scholar

[28] A. Ferancova, L. Heilerova, E. Korgova, S. Silhar, I. Stepanek, J. Labuda, Eur. Food Res. Technol. 219, 416 (2004) http://dx.doi.org/10.1007/s00217-004-1001-710.1007/s00217-004-1001-7Search in Google Scholar

[29] G. Ziyatdinova, J. Galandova, J. Labuda, J. Int. J. Electrochem. Sci. 3, 223 (2008) Search in Google Scholar

[30] Y. Yardim, J. Food Sci. 77, C408 (2012) http://dx.doi.org/10.1111/j.1750-3841.2011.02609.x10.1111/j.1750-3841.2011.02609.xSearch in Google Scholar PubMed

[31] V. Vyskocil, J. Labuda, J. Barek, Anal. Bioanal. Chem. 397, 233 (2010) http://dx.doi.org/10.1007/s00216-010-3517-y10.1007/s00216-010-3517-ySearch in Google Scholar PubMed

Published Online: 2014-2-21
Published in Print: 2014-5-1

© 2014 Versita Warsaw

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Downloaded on 29.3.2024 from https://www.degruyter.com/document/doi/10.2478/s11532-014-0516-4/html
Scroll to top button