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Licensed Unlicensed Requires Authentication Published by De Gruyter November 11, 2014

Automated immunoassays for the autoantibodies to carbamylated or citrullinated telopeptides of type I and II collagens

  • Jere Häyrynen , Maija Kärkkäinen , Aulikki Kononoff , Leena Arstila , Pia Elfving , Helena Niinisalo , Elina Savolainen , Hannu Kautiainen , Juha Risteli , Oili Kaipiainen-Seppänen and Marja-Kaisa Koivula EMAIL logo

Abstract

Background: The aim of the study was to describe automated immunoassays for autoantibodies to homocitrulline or citrulline containing telopeptides of type I and II collagen in various disease categories in an early arthritis series.

Methods: Serum samples were collected from 142 patients over 16 years of age with newly diagnosed inflammatory joint disease. All samples were analyzed with an automated inhibition chemiluminescence immunoassay (CLIA) using four different peptide pairs, each consisting of a biotinylated antigen and an inhibiting peptide. Assays were performed with an IDS-iSYS analyzer. Autoantibodies binding to homocitrulline and citrulline containing C-telopeptides of type I (HTELO-I, TELO-I) and type II collagens (HTELO-II, TELO-II) were analyzed.

Results: The mean ratio of HTELO-I inhibition in seropositive and seronegative rheumatoid arthritis (RA) was 3.07 (95% CI 1.41–11.60), p=0.003, and in seropositive and seronegative undifferentiated arthritis (UA) 4.90 (1.85–14.49), p<0.001. The respective mean ratios in seropositive and seronegative RA and UA were in TELO-I 8.72 (3.68–58.01), p<0.001 and 3.13 (1.49–6.16), p=0.008, in HTELO-II 7.57 (3.18–56.60), p<0.001 and 2.97 (1.23–6.69), p=0.037, and in TELO-II 3.01 (1.30–9.51), p=0.002 and 3.64 (1.86–7.65), p=0.008. In reactive arthritis, ankylosing spondylitis, psoriatic arthritis and unspecified spondyloarthritis the inhibition levels were similar to those observed in seronegative RA or UA.

Conclusions: Autoantibodies binding to homocitrulline or citrulline containing telopeptides of type I and II collagen did not differ significantly. They were highest among patients with seropositive disease and they differentiated seropositive and seronegative arthritis.


Corresponding author: Marja-Kaisa Koivula, PhD, Adjunct Professor, Clinical Biochemist, Institute of Diagnostics, Department of Clinical Chemistry, PO Box 5000, 90014 University of Oulu, Oulu, Finland, Phone: +358 40 635 6279, E-mail: ; Northern Finland Laboratory Center NordLab, Oulu University Hospital, Oulu, Finland; and Medical Research Center Oulu, Oulu University Hospital, Oulu, Finland

Acknowledgments

We thank Ms Katja Koukkula for expert technical assistance. This study was supported by the Finnish Society of Clinical Chemistry (MKK).

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

Financial support: None declared.

Employment or leadership: None declared.

Honorarium: None declared.

Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2014-7-3
Accepted: 2014-10-13
Published Online: 2014-11-11
Published in Print: 2015-8-1

©2015 by De Gruyter

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