In order to utilize lignocellulosic biomass as a feedstock for bioethanol, ozone pretreatment was conducted on Japanese cedar sawdust and three other lignocellulosic wastes. Successful lignin degradation was accomplished by ozone pretreatment of the Japanese cedar sawdust and over 90% of polysaccharides were converted to monomeric sugars by enzymatic saccharification. This ozone pretreatment was also effective with other lignocellulosics, such as Hinoki cypress sawdust, lumber and board wastes. Ethanol production by simultaneous saccharification and fermentation of the ozone pretreated Japanese cedar sawdust was also successful. It was shown that ozone pretreatment increases enzymatic susceptibility and enables the production of ethanol from lignocellulosic biomass.
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