Ozone pretreatment of lignocellulosic materials for ethanol production: Improvement of enzymatic susceptibility of softwood

Tomoko Sugimoto 1 , Kengo Magara 1 , Shuji Hosoya 1 , Satoshi Oosawa 2 , Takafumi Shimoda 3 ,  and Kozo Nishibori 3
  • 1 Wood Chemistry Laboratory, Forestry and Forest Products Research Institute, Tsukuba, Japan
  • 2 Daitofujitec Co., Ltd., Yokohama, Japan
  • 3 Yukiguni Maitake Co., Ltd., Minamiuonuma, Japan


In order to utilize lignocellulosic biomass as a feedstock for bioethanol, ozone pretreatment was conducted on Japanese cedar sawdust and three other lignocellulosic wastes. Successful lignin degradation was accomplished by ozone pretreatment of the Japanese cedar sawdust and over 90% of polysaccharides were converted to monomeric sugars by enzymatic saccharification. This ozone pretreatment was also effective with other lignocellulosics, such as Hinoki cypress sawdust, lumber and board wastes. Ethanol production by simultaneous saccharification and fermentation of the ozone pretreated Japanese cedar sawdust was also successful. It was shown that ozone pretreatment increases enzymatic susceptibility and enables the production of ethanol from lignocellulosic biomass.

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Holzforschung is an international scholarly journal that publishes cutting-edge research on the biology, chemistry, physics and technology of wood and wood components. High quality papers about biotechnology and tree genetics are also welcome. Rated year after year as one of the top scientific journals in the category of Pulp and Paper (ISI Journal Citation Index), Holzforschung represents innovative, high quality basic and applied research.