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Licensed Unlicensed Requires Authentication Published by De Gruyter May 29, 2020

Evaluation of α-klotho level in insulin dependent diabetes mellitus (IDDM) children

  • Fariba Tarhani ORCID logo , Ghobad Heidari ORCID logo and Alireza Nezami ORCID logo EMAIL logo

Abstract

Objectives

Reduced levels of α-Klotho is associated with the pathogenesis of various diseases including diabetes. In type I diabetes, decrease in Klotho leads to apoptosis of β-cells of pancreases. The aim of this study was to evaluate the levels of α-Klotho in type I diabetic pediatric patients.

Methods

In this cross-sectional single centered study, 46 patients presenting type I diabetes mellitus (case group) and 78 control group under the age of 12, referred to our clinic were included in our study. Serum levels of soluble Klotho were measured by sandwich ELISA in case and control groups. Statistical analysis was conducted for the data recorded via questionnaire.

Results

Mean age of the patients in the case and control group was 7.65 ± 3.09 and 7 ± 2.37, respectively. Type I diabetes patients had a significant reduction in the levels of serum Klotho, as compared to controls (p<0.001). However, gender and age-based comparison between patient and control group was not significant.

Conclusions

This study reports a significant decrease in the serum levels of α-Klotho in type 1 diabetic patients. Low levels of Klotho can be associated with diabetic nephropathy and other comorbidities in these patients.


Corresponding author: Alireza Nezami, Assistant professor of Pediatrics, Department of Pediatric, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran, E-mail:

  1. Research funding: No funding was secured for this study.

  2. Consent to participate: From the under 16 years old was given by a parent or legal guardian.

  3. Ethical approval: This protocol was approved by the Ethics Committee of Lorestan University of Medical Sciences, after obtaining the necessary permission from the University and the Department of Education of Khorramabad, Lorestan province. All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. No Animals were used for studies that are the basis of this research.

  4. Author contribution: Dr. Alireza Nezami: conceptualized and designed the study, drafted the initial manuscript, and reviewed and revised the manuscript. Dr. Fariba Tarhani: Designed the data collection instruments, collected data, carried out the initial analyses, and reviewed and revised the manuscript. Dr. Ghobad Heidari: Coordinated and supervised data collection, and critically reviewed the manuscript for important intellectual content. All authors approved the final manuscript as submitted and agree to be accountable for all aspects of the work.

  5. Conflict of interest: The authors deny any conflict of interest in any terms or by any means during the study. All the fees provided by research center fund and deployed accordingly.

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Received: 2019-12-21
Accepted: 2020-03-10
Published Online: 2020-05-29

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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