Comprehensive study of serratia peptidase immobilization from Serratia sp. ZF03 onto chitosan nanogels

Navvabeh Salarizadeh 1 , Sadegh Hasannia 2 , Reza Hassan Sajedi 2 , Navid Lamei 3 , Afshin Mohsenifar 4  and Kambiz Akbari Noghabi 4
  • 1 Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran (Islamic Republic of)
  • 2 Department of Biochemistry, Faculty of Biological sciences, Tarbiat Modares University, Tehran, Iran (Islamic Republic of)
  • 3 Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran (Islamic Republic of)
  • 4 National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran (Islamic Republic of)
Navvabeh Salarizadeh, Sadegh Hasannia, Reza Hassan Sajedi, Navid Lamei, Afshin Mohsenifar and Kambiz Akbari Noghabi

Abstract

Objective:

In the present work, we have extended the study and immobilized the metalloprotease enzyme in glutaraldehyde cross-linked chitosan nanogels to scrutinize the enzyme’s features including stability over its soluble free form.

Method:

The immobilized metalloprotease was characterized using scanning electron microscopy (SEM), followed by Fourier transform infrared (FTIR) spectroscopy. The enzyme is optimally active at 50°C and pH range of 8.0–10.

Results:

Thermal stability of the enzyme enhanced when immobilized on the nanogel. After 5 min of incubation at 50°C, immobilized enzymes retained 60% of their original activity, while negligible activity (23%) was observed in the case of the free enzyme.

Conclusion:

The results obtained here provide a powerful demonstration of the benefits of taking the glutaraldehyde cross-linked chitosan matrices to enhance metalloprotease stability. The high stability of the immobilized enzyme serves to improve its performance for possible application on the industrial scale.

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Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.

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