Molecular modulations and influence of acclimation of Ni on acute Ni toxicity in Plectonema boryanum

Alvina Farooqui 1 , Sadaf Mahfooz 2 , Adeeba Shamim 2 , Haris Mohd. Siddiqui 3  and Kavindra Kumar Kesari 4 , 5
  • 1 Department of Bioengineering Integral University Kursi Road, Dasauli, P.O. Bas-ha Lucknow 226026, Uttar Pradesh, India
  • 2 Integral University, Department of Biosciences, Lucknow, India
  • 3 Integral University, Department of Bioengineering, Lucknow, India
  • 4 Jaipur National University Ringgold standard institution, Department of Engineering and Technology, School of Life Science, Jaipur, Rajasthan, India
  • 5 University of Eastern, Department of Environmental Science, Kuopio, Finland
Alvina Farooqui, Sadaf Mahfooz, Adeeba Shamim, Haris Mohd. Siddiqui and Kavindra Kumar Kesari

Abstract

Objective:

In this study, metal induced accumulation of antioxidants, proline and phenol were proposed during acclimation, to provide defense, protection and resistance against oxidative stress on Ni acclimated cyanobacterium.

Methods:

Plectonema boryanum was used as a test organism in the present study and the experiments were performed in two sets (1) cells differentially exposed to Ni (2) cells acclimated with Ni and then further exposed to Ni in the plant tissue culture laboratory.

Results:

Dose-dependent formation of peroxide in Ni-treated cells increased the activity of superoxide dismutase (SOD) and also enhanced accumulations of non-enzymatic antioxidants such as proline and total phenols at low concentrations of Ni. Down regulation of most of the polypeptides at 20 μM of Ni demonstrated the severe toxicity of the metal while a slight up-regulation of peptide depicted its role in metal tolerance. Ni acclimated cells also showed reduction in peroxide and an increase in proline and total phenol after exposure to 4 μM of Ni.

Conclusion:

Our results for the first time demonstrated that accumulation of phenol and proline during the acclimation process could provide tolerance to the cyanobacterium from deleterious effects of the metal stress if it is further exposed to the same metal.

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Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.

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