Antioxidant potential of some natural and semi-synthetic flavonoid derivatives and the extracts from Maclura pomifera (Rafin.) Schneider (osage orange) and its essential oil composition

Ilkay Erdogan Orhan 1 , Fatma Sezer Senol 2 , Betül Demirci 3 , Margita Dvorska 4 , Karel Smejkal 4  and Milan Zemlicka 5
  • 1 Faculty of Pharmacy, Gazi University – Department of Pharmacognosy, Ankara, Turkey, Tel.: +90 312 202 31 86
  • 2 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, Turkey
  • 3 Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir, Turkey
  • 4 Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, 61242 Brno, Czech Republic
  • 5 Department of Pharmacognosy and Botany, The University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice, Slovakia Republic
Ilkay Erdogan Orhan, Fatma Sezer Senol, Betül Demirci, Margita Dvorska, Karel Smejkal and Milan Zemlicka

Abstract

Objective:

The antioxidant potential of various extracts were obtained from the leaf and fruit of Maclura pomifera (Rafin.) Schneider (Moraceae) along with its major isoflavonoids; osajin and pomiferin, their semi-synthetic derivatives; iso-osajin and iso-pomiferin and macluraxanthone, which were assayed.

Methods:

The extracts and compounds were subjected to six experimental models including 2,2′-diphenyl-1-picrylhydrazyl (DPPH), N,N-dimethyl-p-phenylendiamine (DMPD+), and nitric oxide (NO) radical scavenging activity, metal-chelating capacity, ferric- (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) assays by using ELISA methods.

Results:

The fruit aqueous extract exerted higher scavenging activity against DMPD and NO radicals, while the fruit ethyl acetate extract was the most active against DPPH radical (68.61±2.53%). Among the tested compounds, the highest DPPH (91.74±0.26%) and DMPD (30.63±1.31%) radical scavenging effect was observed with macluraxanthone, while pomiferin and iso-pomiferin exhibited better activity than osajin and iso-osajin in all assays except the metal-chelation capacity assay. Phytol was the major compound in both the leaf oils, while the fruit essential oil contained β-caryophyllene as the main component (69.3%).

Discussion and Conclusion:

The current study covers particularly antioxidant capacity of iso-osajin, iso-pomiferin, and macluraxanthone by the aforementioned methods and, among them; pomiferin seems to be a natural possible antioxidant agent.

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Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.

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