Association between MTHFR C677T polymorphism and folate, vitamin B12, homocysteine, and DNA fragmentation in patients with ovarian cancer

Anıl Çağla Özkılıç 1 , Ahmet Çetin 2 , Burcu Bayoğlu 3 , Huriye Balcı 4  and Müjgan Cengiz 3
  • 1 Refik Saydam National Public Health Agency, Istanbul, Turkey
  • 2 Haseki Education and Research Hospital, Department of Obstetrics and Gynecology, Istanbul, Turkey
  • 3 Istanbul University, Cerrahpaşa Medical Faculty, Department of Medical Biology, Istanbul, Turkey, Phone: +90 0212 414 30 00/22657, Fax: +90 0212 586 15 48
  • 4 Istanbul University, Cerrahpaşa Medical Faculty, Fikret Biyal Central Research Laboratory, Istanbul, Turkey
Anıl Çağla Özkılıç, Ahmet Çetin, Burcu Bayoğlu, Huriye Balcı and Müjgan Cengiz

Abstract

Objective:

Methylenetetrahydrofolate reductase (MTHFR) is an important enzyme that regulates the metabolism of methionine and folate. MTHFR C677T polymorphism was reported to be associated with breast and ovarian cancer. The aim of this study was to evaluate the association between the MTHFR C677T (rs1801133) polymorphism and homocysteine, vitamin B12, and folate levels, and DNA fragmentation in patients with ovarian cancer and healthy controls.

Materials and methods:

This case-control study was conducted in Istanbul University Cerrahpasa Medical Faculty. We studied 50 ovarian cancer patients and 54 healthy controls. The MTHFR C677T polymorphism was determined by PCR followed by restriction fragment length polymorphism (RFLP) and agarose gel electrophoresis. DNA fragmentation was assessed by the comet assay. Homocysteine levels were measured by ELISA, whereas vitamin B12 and folate levels were measured by chemiluminescence methods.

Results:

We found no correlation between the MTHFR C677T polymorphism and ovarian cancer. No significant difference was found in homocysteine, folate, and vitamin B12 levels between patient and control groups. Increased DNA fragmentation was detected in patients with ovarian cancer.

Conclusion:

Our findings suggest that MTHFR C677T polymorphism, as well as homocysteine, folic acid, and vitamin B12 levels, are not associated with an increased risk for ovarian cancer.

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Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.

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