Assessment of macroprolactinemia rate in a training and research hospital from Turkey

Emiş Deniz Akbulut 1 , Müjgan Ercan 2 , Serpil Erdoğan 3 , Canan Topçuoğlu 4 , Fatma Meriç Yılmaz 5  and Turan Turhan 6
  • 1 Türkiye Yüksek İhtisas Training and Research Hospital, Department of Biochemistry, Ankara, Ankara, Turkey
  • 2 Aydın Public Health Laboratory, Department of Biochemistry, Aydın, Turkey
  • 3 Ankara Atatürk Training and Research Hospital, Department of Medical Biochemistry, Ankara, Turkey
  • 4 Ankara Numune Training And Research Hospital, Department of Biochemistry, Ankara, Turkey
  • 5 Yıldırım Beyazıt University, Faculty of Medicine, Department of Biochemistry, Ankara, Turkey
  • 6 Ankara Numune Training and Research Hospital, Department of Biochemistry, Ankara, Turkey
Emiş Deniz Akbulut, Müjgan Ercan, Serpil Erdoğan, Canan Topçuoğlu, Fatma Meriç Yılmaz and Turan Turhan

Abstract

Objective:

Macroprolactinemia detection is important to avoid unneccessary tests and overtreatment. High prolactin levels require routine screening and clinicians must be aware of macroprolactinemia frequency encountered with the method in use. In this study we aimed to determine the macroprolactinemia rate in our laboratory.

Methods:

Prolactin results of different patients analysed on two different immunoassay systems within two consecutive years were evaluated. Analyses were performed on Beckman Coulter UniCel® DxI800 and Roche Cobas® e601 immunoassay systems. Samples for macroprolactin analysis were precipitated using polyethylene glycol (PEG) 6000. Post-PEG recovery <40% was defined as positive, 40–60% as gray-zone and >60% as negative for macroprolactin.

Results:

For the samples analysed on DxI800 (n=14,958) hyperprolactinemia frequency was 8.1% (n=1208). One of 138 samples submitted for macroprolactin analysis was positive, while three of them were in the gray-zone. For the samples analysed on Cobas® e601 (n=14,040) hyperprolactinemia frequency was 13.9% (n=1954). Eighteen of 238 samples submitted for macroprolactin analysis were positive, while 21 of them were in the gray-zone.

Conclusion:

A difference was found between two immunoassay systems used in our laboratory in terms of macroprolactinemia rate. However, inability of simultaneous analyses on both systems, lack of evaluation with gel filtration chromatography, and heterophile antibody blocking tube were the limitations.

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Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.

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