Cytogenetic impact of sodium chloride stress on root cells of Vigna radiata L. seedlings

  • 1 Department of Genetics, Faculty of Science, University of Karachi, Karachi 75270, Pakistan
  • 2 Department of Genetics, Faculty of Science, University of Karachi, Karachi, Pakistan
Jabeen FarheenORCID iD: https://orcid.org/0000-0002-0244-7856 and Simeen Mansoor
  • Department of Genetics, Faculty of Science, University of Karachi, Karachi, Pakistan
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Abstract

Objectives

The high salinization stress to seedling is the substantial ecological problem in the ongoing era. It negatively influences the growth that retard mitotic division by enhancing aberrations in nuclear chromatin. In the light of these views, the current work was designed to investigate the response of Vigna seedlings root tip cells to the presence of NaCl ions.

Materials and methods

NM-92 and NM19-19 seeds were imbibed separately in distilled water for 24 h and allowed to grow into 0, 50, 150, 250, and 350 mM NaCl solution for 24 h. Excised root tips were stained, and slides were scored at 100× objective for the mitotic index (MI) and chromosomal aberrations.

Results

Our data demonstrated that as NaCl molarity increased, the MI was declined along with various chromatin abnormalities. The 150 mM of NaCl showed more lagging (69%) of chromosomes during anaphase in NM19-19. The highest stickiness at metaphase stage (68%) was found in 250 mM NaCl in variety NM19-19. However, both varieties were differed non-significantly for c-mitosis that was recorded 99% at 350 mM NaCl concentration.

Conclusions

The NaCl ions toxicity induced various cytological anomalies in seedling roots that adversely affect the growth of Vigna seedlings.

  • 1.

    Tabur S, Demir K. Protective roles of exogenous polyamines on chromosomal aberrations in Hordeum vulgare exposed to salinity. Biologia 2010;65:947–53.

  • 2.

    Çavuşoğlu D, Tabur S, Çavuşoğlu K. Role of Ginkgo biloba L. leaf extract on some physiological and cytogenetical parameters in Allium cepa L. seeds exposed to salt stress. Cytologia 2016;81:207–13.

    • Crossref
    • Export Citation
  • 3.

    Nasri N, Saïdi I, Kaddour R, Lachaâl M. Effect of salinity on germination, seedling growth and acid phosphatase activity in lettuce. Am J Plant Sci 2015;6:57–63.

    • Crossref
    • Export Citation
  • 4.

    Chauhan A, Rajput N, Kumar D, Kumar A, Chaudhry AK. Effect of different salt concentration on seed germination and seedling growth of different varieties of oat (Avena sativa L.). Int J Inf Res Rev 2016;3:2627–32.

  • 5.

    Pekol S, Baloğlu MC, Çelik Altunoğlu Y. Evaluation of genotoxic and cytologic effects of environmental stress in wheat species with different ploidy levels. Turk J Biol 2016;40:580–8.

    • Crossref
    • Export Citation
  • 6.

    Jones MB, Finnan J, Hodkinson TR. Morphological and physiological traits for higher biomass production in perennial rhizomatous grasses grown on marginal land. GCB Bioenergy 2015;7:375–85.

    • Crossref
    • Export Citation
  • 7.

    Dikshit HK, Singh D, Singh A, Jain N, Kumari J, Sharma TR. Utility of adzuki bean [Vigna angularis (Willd.) Ohwi & Ohashi] simple sequence repeat (SSR) markers in genetic analysis of mungbean and related Vigna spp. Afr J Biotechnol 2012;11:13261–8.

  • 8.

    Sony SK, Habib A, Islam MN. Genetic diversity analysis of thirteen mungbean (Vigna radiata (L.) Wilczek) cultivars under RAPD markers. Bangl J Bot 2012;41:169–75.

  • 9.

    Taunk J, Yadav NR, Yadav RC, Kumar R. Genetic diversity among green gram [Vigna radiata (L.) Wilczek] genotypes varying in micronutrient (Fe and Zn) content using RAPD markers. Indian J Biotechnol 2012;11:48–53.

  • 10.

    Government of Pakistan (2015–2016). Ministry of national food security and research (NFS & R), 2015–2016. FCA Working Paper Draft 27 April 15 Final.pdf.

  • 11.

    Farheen J, Mansoor S, Abideen Z. Application of salicylic acid improved growth, photosynthesis pigments and oxidative stability in mungbean seedlings at salt stress. Pak J Bot 2018;50:901–12.

  • 12.

    Kiełkowska A. Allium cepa root meristem cells under osmotic (sorbitol) and salt (NaCl) stress in vitro. Acta Bot Croat 2017;76:146–53.

    • Crossref
    • Export Citation
  • 13.

    Marakli S, Temel A, Gozukirmizi N. Salt stress and homobrassinosteroid interactions during germination in barley roots. Not Bot Horti Agrobo 2014;42:446–52.

    • Crossref
    • Export Citation
  • 14.

    Çavuşoğlu K, Cadıl S, Çavuşoğlu D. Role of potassium nitrate (KNO3) in alleviation of detrimental effects of salt stress on some physiological and cytogenetical parameters in Allium cepa L. Cytologia 2017;82:279–86.

    • Crossref
    • Export Citation
  • 15.

    Singh D, Roy BK. Salt stress affects mitotic activity and modulates antioxidant systems in onion roots. Braz J Bot 2016;39:67–76.

    • Crossref
    • Export Citation
  • 16.

    Kaur M, Soodan RK, Katnoria JK, Bhardwaj R, Pakade YB, Nagpal AK. Analysis of physico-chemical parameters, genotoxicity and oxidative stress inducing potential of soils of some agricultural fields under rice cultivation. Trop Plant Res 2014;1:49–61.

  • 17.

    Sharma AK, Sharma A. Chromosome technique a manual. Chur, Switzerland: Harwood Academic, 1994;305–6.

  • 18.

    Jairajpuri M, Raval R, Patel K. Chromosomal aberrations in root meristems of Allium cepa L. induced by dyeing industrial effluent. Int J Multidiscip Res Dev 2016;3:272–5.

  • 19.

    Steel RG, Torrie JH. Principles and procedures of statistics: a biometrical approach. 2nd ed. Singapore: McGraw Hill Book Co., 1984.

  • 20.

    Khan MA, Shirazi MU, Shereen A, Mujtaba SM, Khan MA, Mumtaz S, et al. Identification of some wheat (Triticum aestivum L.) lines for salt tolerance on the basis of growth and physiological characters. Pak J Bot 2017;49:397–403.

  • 21.

    Stavridou E, Hastings A, Webster RJ, Robson PR. The impact of soil salinity on the yield, composition and physiology of the bioenergy grass Miscanthus X giganteus. GCB Bioenergy 2017;9:92–104.

    • Crossref
    • Export Citation
  • 22.

    Lubini G, Fachinetto JM, Laughinghouse HD, Paranhos JT, Silva AC, Tedesco SB. Extracts affecting mitotic division in root-tip meristematic cells. Biologia 2008;63:647–51.

  • 23.

    Jităreanu A, Pădureanu S, Tătăringă G, Tuchiluş C, Stănescu U. Evaluation of phytotoxic and mutagenic effects of some cinnamic acid derivatives using the Triticum test. Turk J Biol 2013;37:748–56.

    • Crossref
    • Export Citation
  • 24.

    Tabur S, Demir K. Cytogenetic response of 24-epibrassinolide on the root meristem cells of barley seeds under salinity. Plant Growth Regul 2009;58:119–23.

    • Crossref
    • Export Citation
  • 25.

    Utani K, Kohno Y, Okamoto A, Shimizu N. Emergence of micronuclei and their effects on the fate of cells under replication stress. PLoS One 2010;5:e10089.

    • Crossref
    • PubMed
    • Export Citation
  • 26.

    Leme DM, Marin-Morales MA. Allium cepa test in environmental monitoring: a review on its application. Mutat Res 2009;682:71–81.

    • Crossref
    • PubMed
    • Export Citation
  • 27.

    Yazdani M, Mahdieh M. Salinity induced apoptosis in root meristematic cells of rice. Int J Biosci Biochem Bioinforma 2012;2:40–3.

  • 28.

    Mudgal M, Madaan N, Mudgal A. Biochemical mechanisms of salt tolerance in plants: a review. Int J Bot 2010;6:136–43.

    • Crossref
    • Export Citation
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Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.

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