Evaluation ofTrichoderma atroviride and Trichoderma citrinoviride growth profiles and their potentials as biocontrol agent and biofertilizer

Yüksel Gezginhttp://orcid.org/https://orcid.org/0000-0001-5812-1882 1 , Derya Maral Gül 2 , Seçil Sözer Şenşatar 2 , Can Uraz Kara 2 , Sayit Sargın 2 , Fazilet Vardar Sukan 2  and Rengin Eltem 2
  • 1 Department of Bioengineering, Faculty of Engineering, Ege University, 35100 Bornova-Izmir, Turkey
  • 2 Department of Bioengineering, Faculty of Engineering, Ege University, Bornova-Izmir, Turkey
Yüksel GezginORCID iD: https://orcid.org/0000-0001-5812-1882, Derya Maral Gül
  • Department of Bioengineering, Faculty of Engineering, Ege University, Bornova-Izmir, Turkey
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, Seçil Sözer Şenşatar
  • Department of Bioengineering, Faculty of Engineering, Ege University, Bornova-Izmir, Turkey
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, Can Uraz Kara
  • Department of Bioengineering, Faculty of Engineering, Ege University, Bornova-Izmir, Turkey
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, Sayit Sargın
  • Department of Bioengineering, Faculty of Engineering, Ege University, Bornova-Izmir, Turkey
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, Fazilet Vardar Sukan
  • Department of Bioengineering, Faculty of Engineering, Ege University, Bornova-Izmir, Turkey
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and Rengin Eltem
  • Department of Bioengineering, Faculty of Engineering, Ege University, Bornova-Izmir, Turkey
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Abstract

Background

Two Trichoderma species (T. atroviride and T. citrinoviride) were evaluated for their potential to have Trichoderma-based biological control agent and biofertilizer properties.

Materials and Methods

Eight Trichoderma spp. were identified by molecular methods. The mycoparasitic activities against different phytopathogenic fungi and their capacity to produce extracellular lytic enzymes were investigated. Furthermore, indole-3-acetic acid production and phosphate solubilization capabilities of Trichoderma spp. were evaluated together with the effect of some physical parameters and different carbon:nitrogen sources on mycelial growth and conidium production.

Results and Discussion

All strains exhibited lytic enzymes and indole-3 acetic acid production as well as phosphate solubility characteristics. Trichoderma citrinoviride demostrated more mycoparasitic activity against Fusarium oxysporium than T. atroviride whereas T. atroviride was found more effective against Rhizoctonia solani. In particular, at lower temperatures, conidium production of T. atroviride strains were significantly higher than T. citrinoviride strains. Both strains grew well on all carbon sources tested. The effect of organic nitrogen sources on growth were notably higher than inorganic nitrogen sources.

Conclusion

The results provided valuable insight in both the highest mycelial growth and conidia production conditions of these cultures for further similar studies related to development of Trichoderma-based new commercial biological control and biofertilizer formulations in different agro-climatic regions.

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Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.

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