The effect of Diplotaenia turcica root extract in streptozotocin-induced diabetic rats

  • 1 Vocational School of H ealth Services, Yuzuncu Yil University, Van, Turkey
  • 2 Faculty of Veterinary Medicine, Atatürk University, Department of Pathology, Erzurum, Turkey
  • 3 Department of Biochemistry, Faculty of Veterinary Medicine, University of Van Yuzuncu Yil, Van, Turkey
Uğur ÖzdekORCID iD: https://orcid.org/0000-0002-0709-1545, Serkan Yıldırım and Yeter Değer
  • Department of Biochemistry, Faculty of Veterinary Medicine, University of Van Yuzuncu Yil, Van, Turkey
  • Email
  • Search for other articles:
  • degruyter.comGoogle Scholar

Abstract

Background

Diplotaenia turcica has been used traditionally to diabetes treatment. In this study, the effects of D. turcica root extract (DT) on diabetes mellitus induced by streptozotocin (STZ) were investigated.

Materials and methods

In this study, 78 male rats were used, rats were divided into 9 groups randomly. In diabetic groups, STZ was given a single dose of 45 mg/kg by intraperitoneally. DT (50, 100 and 200 mg/kg) and glibenclamide (5 mg/kg) were given by orally. Blood and pancreas tissue samples were taken for biochemical and pathological tests.

Results

It was found that glucose levels decreased, and insulin levels increased in the treatment groups compared with the diabetes group. In addition, only in 200 mg/kg DT dose group was found to decrease HbA1c levels. Pancreatic tissue analysis showed that MDA levels decreased and GSH levels and CAT, SOD, GSH-Px and GSH-R activities increased in diabetic rats treated with DT. Histopathological and immunohistochemical examinations of the pancreas showed significant improvements in the treatment with DT.

Conclusion

These results clearly show the antioxidant property of DT. The findings of this study showed that increased doses of DT may have a therapeutic effect on STZ-induced pancreatic damage.

  • 1.

    Gomathi D, Ravikumar G, Kalaiselvi M, Devaki K, Uma C. Efficacy of Evolvulus alsinoides (L.) L. on insulin and antioxidants activity in pancreas of streptozotocin induced diabetic rats. J Diabetes Metab Disord 2013;12:39–44.

    • Crossref
    • Export Citation
  • 2.

    Genet S, Kale RK, Baquer NZ. Alterations in antioxidant enzymes and oxidative damage in experimental diabetic rat tissues: effect of vanadate and fenugreek (Trigonella foenum graecum). Mol Cell Biochem 2002;236:7–12.

    • Crossref
    • PubMed
    • Export Citation
  • 3.

    Jouad H, Maghrani M, Eddouks M. Hypoglycaemic effect of Rubus fructicosis L. and Globularia alypum L. in normal and streptozotocin-induced diabetic rats. J Ethnopharmacol 2002;81:351–6.

    • Crossref
    • PubMed
    • Export Citation
  • 4.

    Krishnasamy G, Muthusamy K, Chellappan DR, Subbiah N. Antidiabetic, antihyperlipidaemic, and antioxidant activity of Syzygium densiflorum fruits in streptozotocin and nicotinamide-induced diabetic rats. Pharm Biol 2016;54:1716–26.

    • Crossref
    • PubMed
    • Export Citation
  • 5.

    Bulduk B, Kılınç DK. The effect of daily application of Crataegus oxyacantha (Hawthorn) on the values of ECG in rats. Van Vet J 2013;24:77–81.

  • 6.

    Farzami B, Ahmadvand D, Vardasbi S, Majin F, Khaghani S. Induction of insulin secretion by a component of Urtica dioica leave extract in perifused Islets of Langerhans and its in vivo effects in normal and streptozotocin diabetic rats. J Ethnopharmacol 2003;89:47–53.

    • Crossref
    • PubMed
    • Export Citation
  • 7.

    Patel D, Prasad S, Kumar R, Hemalatha S. An overview on antidiabetic medicinal plants having insulin mimetic property. Asian Pac J Trop Biomed 2012;2:320–30.

    • Crossref
    • PubMed
    • Export Citation
  • 8.

    Özdek U, Başbuğan Y, Yıldırım S, Fırat M, Değer Y. Activity, acute and sub-acute toxicity and safety assessment of the hydroalcoholic root extract of Diplotaenia turcica. Indian J Anim Res 2018;52:1688–94.

  • 9.

    Yanardağ R, Bolkent Ş, Tabakoğlu-Oğuz A, Özsoy-Saçan Ö. Effects of Petroselinum crispum extract on pancreatic B cells and blood glucose of streptozotocin-induced diabetic rats. Biol Pharm Bull 2003;26:1206–10.

    • Crossref
    • PubMed
    • Export Citation
  • 10.

    Jagtap A, Patil P. Antihyperglycemic activity and inhibition of advanced glycation end product formation by Cuminum cyminum in streptozotocin induced diabetic rats. Food Chem Toxicol 2010;48:2030–6.

    • Crossref
    • PubMed
    • Export Citation
  • 11.

    Akhlaghi F, Rajaei Z, Hadjzadeh MA, İranshahi M, Mahdi A. Antihyperglycemic effect of asafoetida (Ferula assafoetida Oleo-Gum-Resin) in streptozotocin-induced diabetic rats. World Appl Sci J 2012;17:157–62.

  • 12.

    Abou Khalil NS, Abou-Elhamd AS, Wasfy SI, El Mileegy IM, Hamed MY, Ageely HM. Antidiabetic and antioxidant impacts of desert date (Balanites aegyptiaca) and parsley (Petroselinum sativum) aqueous extracts: lessons from experimental rats. J Diabetes Res 2016;2016:1–10.

  • 13.

    Uce İ, Tunçtürk M. Wild plants which naturally grown and widely used in Hakkari. Res J Biol Sci 2014;7:21–5.

  • 14.

    Kaval I, Behçet L, Cakilcioglu U. Ethnobotanical study on medicinal plants in Geçitli and its surrounding (Hakkari-Turkey). J Ethnopharmacol 2014;155:171–84.

    • Crossref
    • Export Citation
  • 15.

    Farkhad NK, Farokhi F, Tukmacki A. Hydro-alcoholic extract of the root of Prangos ferulacea (L.) Lindl can improve serum glucose and lipids in alloxan-induced diabetic rats. Avicenna J Phytomed 2012;2:179–87.

    • PubMed
    • Export Citation
  • 16.

    Kumar V, Jain P, Rathore K, Ahmed Z. Biological evaluation of Pupalia lappacea for antidiabetic, antiadipogenic, and hypolipidemic activity both in vitro and in vivo. Scientifica (Cairo) 2016;2016:1–10.

  • 17.

    Andrade-Cetto A. Hypoglycemic effect of Smilax moranensis root on N5-STZ diabetic rats. Pharmacologyonline 2011;1:111–5.

  • 18.

    Giribabu N, Kumar KE, Rekha SS, Muniandy S, Salleh N. Chlorophytum borivilianum root extract maintains near normal blood glucose, insulin and lipid profile levels and prevents oxidative stress in the pancreas of streptozotocin-induced adult male diabetic rats. Int J Res Med Sci 2014;11:1172–84.

  • 19.

    Kurcer Z, Karaoglu D. The use of alloxan and streptozotocin in experimental diabetes models. Turk J Endocrinol Metab 2012;16:34–41.

  • 20.

    Pabbidi RM, Cao D-S, Parihar A, Pauza ME, Premkumar LS. Direct role of streptozotocin in inducing thermal hyperalgesia by enhanced expression of transient receptor potential vanilloid 1 in sensory neurons. Mol Pharmacol 2008;73:995–1004.

    • Crossref
    • PubMed
    • Export Citation
  • 21.

    Noyan T, Balahoroğlu R, Kömüroğlu U. The effects of vitamin A, E, and C combined insulin treatments on the antioxidant enzymes in the diabetic rats. J Turk Clin Biochem 2004;2:113–9.

  • 22.

    Attanayake A, Jayatilaka K, Pathirana C, Mudduwa L. Antihyperglycemic activity of Coccinia grandis (L.) Voigt in streptozotocin induced diabetic rats. Indian J Tradit Know 2015;14:376–81.

  • 23.

    Govindappa M. A review on role of plant (s) extracts and its phytochemicals for the management of diabetes. J Diabetes Metab 2015;6:1–38.

  • 24.

    Pimenov M, Kljuykov E, Degtjareva G. Survey of the genus Diplotaenia (Umbelliferae), with description of two new species from Turkey. Willdenowia 2011;41:67–74.

    • Crossref
    • Export Citation
  • 25.

    Soltani BK, Kafash FN, Farokhi F, Togmechi A. Effects of hydro-alcoholic extract of Prangos ferulacea (L.) Lindle on histopathology of pancreas and diabetes treatment in STZ-induced diabetic rats. Avicenna J Phytomed 2012;2:31–8.

  • 26.

    Noriega-Cisneros R, Ortiz-Avila O, Esquivel-Gutiérrez E, Clemente-Guerrero M, Manzo-Avalos S, Salgado-Garciglia R, et al. Hypolipidemic activity of Eryngium carlinae on streptozotocin-induced diabetic rats. Biochem Res Int 2012:1–5.

  • 27.

    Ramadan BK, Schaalan MF, Tolba AM. Hypoglycemic and pancreatic protective effects of Portulaca oleracea extract in alloxan induced diabetic rats. BMC Complement Altern Med 2017;17:37–46.

    • Crossref
    • PubMed
    • Export Citation
  • 28.

    Karaca T, Yoruk M, Yoruk IH, Uslu S. Effects of extract of green tea and ginseng on pancreatic beta cells and levels of serum glucose, insulin, cholesterol and triglycerides in rats with experimentally streptozotocin-induced diabetes: a histochemical and immunohistochemical study. J Anim Vet Adv 2010;9:102–7.

    • Crossref
    • Export Citation
  • 29.

    Perez-Gutierrez RM, Juarez VA, Sauceda JV, Anaya-Sosa I. In vitro and in vivo antidiabetic and antiglycation properties of Apium graveolens in type 1 and 2 diabetic rats. Int J Pharmacol 2014;10:368–79.

    • Crossref
    • Export Citation
  • 30.

    Roy V, Chenkual L, Gurusubramanian G. Mallotus roxburghianus modulates antioxidant responses in pancreas of diabetic rats. Acta Histochemica 2016;118:152–63.

    • Crossref
    • PubMed
    • Export Citation
  • 31.

    Díaz-Flores M, Angeles-Mejia S, Baiza-Gutman L, Medina-Navarro R, Hernández-Saavedra D, Ortega-Camarillo C, et al. Effect of an aqueous extract of Cucurbita ficifolia Bouché on the glutathione redox cycle in mice with STZ-induced diabetes. J Ethnopharmacol 2012;144:101–8.

    • Crossref
    • PubMed
    • Export Citation
  • 32.

    Gupta R, Sharma AK, Sharma MC, Gupta RS. Antioxidant activity and protection of pancreatic β-cells by embelin in streptozotocin-induced diabetes. J Diabetes 2012;4:248–56.

    • Crossref
    • PubMed
    • Export Citation
  • 33.

    Fathy SM and Drees EA. Protective effects of Egyptian cloudy apple juice and apple peel extract on lipid peroxidation, antioxidant enzymes and inflammatory status in diabetic rat pancreas. BMC Complement Altern Med 2016;16:1–14.

  • 34.

    Jdir H, Kolsi RBA, Zouari S, Hamden K, Zouari N, Fakhfakh N. The cruciferous Diplotaxis simplex: phytochemistry analysis and its protective effect on liver and kidney toxicities, and lipid profile disorders in alloxan-induced diabetic rats. Lipids Health Dis 2017;16:100–9.

    • Crossref
    • PubMed
    • Export Citation
  • 35.

    Adewole SO and Caxton-Martins EA. Morphological changes and hypoglycemic effects of Annona muricata Linn.(annonaceae) leaf aqueous extract on pancreatic β-cells of streptozotocin-treated diabetic rats. Afr J Biomed Res 2006;9:173–87.

  • 36.

    Shukla R, Padhye S, Modak M, Ghaskadbi SS, Bhonde RR. Bis (quercetinato) oxovanadium IV reverses metabolic changes in streptozotocin-induced diabetic mice. Rev Diabet Stud 2007;4:33–43.

    • Crossref
    • PubMed
    • Export Citation
  • 37.

    Karabulut H, Gülay MŞ. Antioxidants. Veterinary J Mehmet Akif Ersoy University 2016;1:65–76.

  • 38.

    Yang J, Cherian MG. Protective effects of metallothionein on streptozotocin-induced diabetes in rats. Life Sci 1994;55:43–51.

    • Crossref
    • PubMed
    • Export Citation
  • 39.

    Arulselvan P and Subramanian SP. Beneficial effects of Murraya koenigii leaves on antioxidant defense system and ultra structural changes of pancreatic β-cells in experimental diabetes in rats. Chem Biol Interact 2007;165:155–64.

    • Crossref
    • PubMed
    • Export Citation
  • 40.

    Yaman T, Uyar A, Celik I, Alkan EE, Keles OF, Yener Z. Histopathological and ımmunohistochemical study of antidiabetic effects of Heracleum persicum extract ın experimentally diabetic rats. Indian J Pharm Educ 2017;51:450–7.

    • Crossref
    • Export Citation
Purchase article
Get instant unlimited access to the article.
$42.00
Log in
Already have access? Please log in.


or
Log in with your institution

Journal + Issues

Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.

Search