Effects of topiramate on adipocyte differentiation and gene expression of certain carbonic anhydrase isoenzymes

Ayşegül Sümerhttp://orcid.org/https://orcid.org/0000-0002-9707-5716 1 , Eşref Edip Keha 2 , Ahmet Menteşe 3  and Ahmet Alver 2
  • 1 Department of Nursing, School of Health Services, Recep Tayyip Erdogan University, 53100 Rize, Turkey
  • 2 Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
  • 3 Program of Medical Laboratory Techniques, Vocational School of Health Sciences, Karadeniz Technical University, Trabzon, Turkey
Ayşegül SümerORCID iD: https://orcid.org/0000-0002-9707-5716, Eşref Edip Keha
  • Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
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, Ahmet Menteşe
  • Program of Medical Laboratory Techniques, Vocational School of Health Sciences, Karadeniz Technical University, Trabzon, Turkey
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  • degruyter.comGoogle Scholar
and Ahmet Alver
  • Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
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  • degruyter.comGoogle Scholar

Abstract

Background

Topiramate, an antiepileptic drug, besides its known effects in neurons, is a carbonic anhydrase inhibitor and causes weight loss in treated subjects, but, the mechanisms of weight loss effect have not been yet identified..

Objective

In this study, effects of topiramate on 3T3-L1 adipocyte differentiation and expressions of carbonic anhydrase I, II, III, VA, VB were investigated to determine possible roles of carbonic anhydrase isoenzymes and adipocytes in weight loss effect of topiramate.

Material and methods

Adipocyte differentiation was detected by Oil Red O staining. Carbonic anhydrase I, II, III, VA, VB expressions were determined by Real-Time PCR method.

Results

It was found that topiramate induced adipogenesis at all concentrations (p < 0.0001). CA expressions increased significantly with adipogenesis (p = 0.0001). Topiramate stimulated adipogenesis in 3T3-L1 cells and reduced CA II, VA and VB and, an increased CA III expression was observed at low concentrations. CA II, VA and, VB expressions were found increased and CA III expression decreased at high topiramate concentrations.

Conclusion

It was concluded that changes in CA III expression were consistent with its reduction in obesity, alterations of CA II, VA and VB expressions could be attributed to compensation mechanism of cells.

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Turkish Journal of Biochemistry (TJB), official journal of Turkish Biochemical Society, is issued electronically every 2 months. The main aim of the journal is to support the research and publishing culture by ensuring that every published manuscript has an added value and thus providing international acceptance of the “readability” of the manuscripts published in the journal.

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