Identification and characterization of novel short-type BmPGRP-S4 from the silkworm, Bombyx mori, involved in innate immunity

Qiang Wang
  • School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, PR China
  • Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China
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, Meijia Ren
  • Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China
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, Xiaoyong Liu
  • Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China
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, Hengchuan Xia
  • Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China
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and Keping Chen
  • Corresponding author
  • Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China
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Abstract

Peptidoglycan recognition proteins (PGRPs) are pattern recognition receptors that can recognize bacterial peptidoglycans and trigger the innate immune response of insects. Here, we identified and characterized a novel short-type Bombyx mori peptidoglycan recognition proteins short-4 (BmPGRP-S4) in a lepidopteran insect, Bombyx mori. BmPGRP-S4 exhibited a cDNA sequence length of 600 bp, encoding 199 aa with a protein molecular weight of 22 kDa. Multiple sequence alignment revealed that BmPGRP-S4 contains a conserved PGRP domain. Quantitative real-time polymerase chain reaction analysis showed that BmPGRP-S4 is highly expressed in the early developmental stages of silkworm larvae and presents tissue-specific expression in hemocytes. Interestingly, BmPGRP-S4 expression is significantly induced by bacterial infection in the midgut, fat body, and hemocytes. Furthermore, a dual luciferase reporter gene assay revealed that BmPGRP-S4 can activate the expression of the antimicrobial peptide genes lebocin, moricin, cecropin D, cecropin B, and attacin. Taken together, these results suggest that BmPGRP-S4 plays an important role in the innate immune response of silkworms.

    • Supplementary Material
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A Journal of Biosciences: Zeitschrift für Naturforschung C (ZNC) is an international scientific journal for the emerging field of natural and natural-like products. ZNC publishes original research on the isolation, bio-chemical synthesis and bioactivities of natural products, their biochemistry, pharmacology, biotechnology, and biological activity and innovative developed computational methods for predicting their structure and/or function.

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