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Licensed Unlicensed Requires Authentication Published by De Gruyter October 11, 2019

Identification and characterization of novel short-type BmPGRP-S4 from the silkworm, Bombyx mori, involved in innate immunity

  • Qiang Wang , Meijia Ren , Xiaoyong Liu , Hengchuan Xia and Keping Chen EMAIL logo

Abstract

Peptidoglycan recognition proteins (PGRPs) are pattern recognition receptors that can recognize bacterial peptidoglycans and trigger the innate immune response of insects. Here, we identified and characterized a novel short-type Bombyx mori peptidoglycan recognition proteins short-4 (BmPGRP-S4) in a lepidopteran insect, Bombyx mori. BmPGRP-S4 exhibited a cDNA sequence length of 600 bp, encoding 199 aa with a protein molecular weight of 22 kDa. Multiple sequence alignment revealed that BmPGRP-S4 contains a conserved PGRP domain. Quantitative real-time polymerase chain reaction analysis showed that BmPGRP-S4 is highly expressed in the early developmental stages of silkworm larvae and presents tissue-specific expression in hemocytes. Interestingly, BmPGRP-S4 expression is significantly induced by bacterial infection in the midgut, fat body, and hemocytes. Furthermore, a dual luciferase reporter gene assay revealed that BmPGRP-S4 can activate the expression of the antimicrobial peptide genes lebocin, moricin, cecropin D, cecropin B, and attacin. Taken together, these results suggest that BmPGRP-S4 plays an important role in the innate immune response of silkworms.

Award Identifier / Grant number: 81502621

Award Identifier / Grant number: 81502088

Award Identifier / Grant number: 31872425

Funding statement: This work was supported by the China Postdoctoral Science Special Foundation (2017M5654), the National Natural Science Foundation of China (81502621 and 81502088, 31872425), and the Postdoctoral Science Foundation of China (2015M571678).

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2019-0093).


Received: 2019-05-07
Revised: 2019-09-01
Accepted: 2019-09-12
Published Online: 2019-10-11
Published in Print: 2020-01-28

©2020 Walter de Gruyter GmbH, Berlin/Boston

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