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Biological Chemistry

Editor-in-Chief: Brüne, Bernhard

Editorial Board: Buchner, Johannes / Lei, Ming / Ludwig, Stephan / Thomas, Douglas D. / Turk, Boris / Wittinghofer, Alfred


IMPACT FACTOR 2018: 3.014
5-year IMPACT FACTOR: 3.162

CiteScore 2018: 3.09

SCImago Journal Rank (SJR) 2018: 1.482
Source Normalized Impact per Paper (SNIP) 2018: 0.820

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1437-4315
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Volume 385, Issue 10

Issues

Generation of glycogen- and albumin-producing hepatocyte-like cells from embryonic stem cells

Gabriela Kania / Przemyslaw Blyszczuk / Andrea Jochheim
  • Hannover Medical School, Center of Internal Medicine, Department of Gastroenterology, Hepatology and Endocrinology, D-30625 Hannover, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Michael Ott
  • Hannover Medical School, Center of Internal Medicine, Department of Gastroenterology, Hepatology and Endocrinology, D-30625 Hannover, Germany
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Anna M. Wobus
Published Online: 2005-06-01 | DOI: https://doi.org/10.1515/BC.2004.123

Abstract

We present a novel two-step protocol for the differentiation of embryonic stem (ES) cells into the hepatic lineage. Differentiated hepatocyte-like cells express genes and proteins characteristic for endodermal and hepatic cells and acquire a functional hepatic phenotype as demonstrated by albumin secretion and glycogen storage. During differentiation, α-fetoprotein, albumin, transthyretin, α-1-antitrypsin, cytochrome P450 subunits 2b9 and 2b13 and tyrosine aminotransferase transcripts are upregulated. Quantitative RT-PCR data revealed a fetal hepatic phenotype corresponding to day 13–14 of liver development. Terminally differentiated hepatocyte-like cells show a bi-nucleated, cuboidal morphology labeled by albumin, α-1-antitrypsin, liver amylase, dipeptidyl peptidase IV, c-met and cytokeratin 18.

ES-derived intermediate cell types transiently and partially co-express nestin with albumin and α-fetoprotein, respectively, but not cytokeratin 19. This finding suggests an ES-derived potential hepatic progenitor cell type, which is partially nestin-, albumin- and α-fetoproteinpositive, but cytokeratin 19-negative.

Keywords: albumin; α-fetoprotein; embryonic stem cells; hepatic differentiation; mouse; nestin

About the article

Received: June 30, 2004

Accepted: August 20, 2004

Published Online: 2005-06-01

Published in Print: 2004-10-01


Citation Information: Biological Chemistry, Volume 385, Issue 10, Pages 943–953, ISSN (Print) 1431-6730, DOI: https://doi.org/10.1515/BC.2004.123.

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