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Journal of Basic and Clinical Physiology and Pharmacology

Editor-in-Chief: Horowitz, Michal

Editorial Board: Das, Kusal K. / Epstein, Yoram / S. Gershon MD, Elliot / Haim, Abraham / Kodesh , Einat / Kohen, Ron / Lichtstein, David / Maloyan, Alina / Mechoulam, Raphael / Roth, Joachim / Schneider, Suzanne / Shohami, Esther / Sohmer, Haim / Yoshikawa, Toshikazu

CiteScore 2016: 1.01

SCImago Journal Rank (SJR) 2016: 0.349
Source Normalized Impact per Paper (SNIP) 2016: 0.495

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Volume 26, Issue 5


Effect of Saraca asoca (Asoka) on estradiol-induced keratinizing metaplasia in rat uterus

Adangam Purath Shahid / Sasidharan Salini
  • Department of Biochemistry, Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India
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/ Nanu Sasidharan / Jose Padikkala
  • Department of Biochemistry, Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India
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/ Achuthan Chathrattil Raghavamenon
  • Department of Biochemistry, Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India
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/ Thekkekara Devassy Babu
  • Corresponding author
  • Department of Biochemistry, Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala, India
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Published Online: 2015-04-25 | DOI: https://doi.org/10.1515/jbcpp-2014-0124


Background: Estrogen-mediated uterus endometrium instability is considered as one of the etiological factors in dysfunctional uterine bleeding (DUB) and uterine cancer. Saraca asoca (Family: Fabaceae) and its fermented preparation, Asokarishta, are extensively used as uterine tonic to treat gynecological disorders in Ayurveda. The present study evaluated the effect of S. asoca (Asoka) on estrogen-induced endometrial thickening of rat uterus.

Methods: Endometrial thickening was induced by intraperitoneal injection of estradiol (20 μg/kg b.wt) to 8-day-old immature rats for alternate 5 days. Methanolic extract (200 mg/kg b. wt) from S. asoca bark was given orally along with estradiol. Uterus endometrial thickening was analyzed histopathologically and serum estrogen level by radioimmunoassay (RIA). Cyclooxygenase (COX-2) expression in rat uterus was also estimated by Western blot. Anti-inflammatory activity of the extract was analyzed by formalin- and carrageenan-elicited paw edema models in mouse.

Results: Uterus endometrium proliferation and keratinized metaplasia with seven to eight stratified epithelial layers on day 16 was observed in rats administered with estradiol. Treatment with S. asoca reduced the thickening to two to four layers and the serum estrogen level diminished significantly to 82.9±12.87 pg/mL compared to rats administered with estrogen alone (111.2±10.68 pg/mL). A reduction of formalin- and carrageenan-induced paw edema in mouse by S. asoca extract was observed. Lower level of lipopolysaccharides (LPS)-induced COX-2 enzyme in rat uterus by the extract further confirms its anti-inflammatory activity.

Conclusions: Present study reveals the antiproliferative and antikeratinizing effects of S. asoca in uterus endometrium possibly through its anti-estrogenic and anti-inflammatory properties.

Keywords: anti-estrogenic; anti-inflammatory; cornification; metaplasia; Saraca asoca (Asoka); uterus endometrium


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About the article

Corresponding author: Thekkekara Devassy Babu, Department of Biochemistry, Amala Cancer Research Centre, Amala Nagar, Thrissur 680 555, Kerala, India, Phone: 919495739939, E-mail:

Received: 2014-12-01

Accepted: 2015-03-11

Published Online: 2015-04-25

Published in Print: 2015-09-01

Citation Information: Journal of Basic and Clinical Physiology and Pharmacology, Volume 26, Issue 5, Pages 509–515, ISSN (Online) 2191-0286, ISSN (Print) 0792-6855, DOI: https://doi.org/10.1515/jbcpp-2014-0124.

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