Jump to ContentJump to Main Navigation
Show Summary Details
More options …

Journal of Pediatric Endocrinology and Metabolism

Editor-in-Chief: Kiess, Wieland

Ed. by Bereket, Abdullah / Darendeliler, Feyza / Dattani, Mehul / Gustafsson, Jan / Luo, Fei Hong / Mericq, Veronica / Ogata, Tsutomu / Toppari, Jorma

12 Issues per year


IMPACT FACTOR 2017: 1.086

CiteScore 2017: 1.07

SCImago Journal Rank (SJR) 2017: 0.465
Source Normalized Impact per Paper (SNIP) 2017: 0.580

Online
ISSN
2191-0251
See all formats and pricing
More options …
Volume 27, Issue 7-8

Issues

Serum paraoxonase activity and oxidative stress and their relationship with obesity-related metabolic syndrome and non-alcoholic fatty liver disease in obese children and adolescents

Emel Torun
  • Corresponding author
  • Department of Pediatrics, Bezmialem Vakif University Hospital, Adnan Menderes Avenue, P.K.: 3409 Fatih, Istanbul
  • Email
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Selim Gökçe
  • Department of Pediatric Gastroenterology, Bezmialem Vakif University Medical Faculty, Istanbul
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ lker Tolga Ozgen
  • Department of Pediatric Endocrinology and Metabolism, Bezmialem Vakif University Medical Faculty, Istanbul
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
/ Sinem Aydın / Yasar Cesur
  • Department of Pediatric Endocrinology and Metabolism, Bezmialem Vakif University Medical Faculty, Istanbul
  • Other articles by this author:
  • De Gruyter OnlineGoogle Scholar
Published Online: 2014-03-29 | DOI: https://doi.org/10.1515/jpem-2013-0337

Abstract

Objective: Oxidative stress has been reported to be involved in the pathogenesis of metabolic disorders related with obesity. The aim of the study was to investigate the association of oxidative stress and paraoxonase activities with non-alcoholic fatty liver disease (NAFLD) as well as metabolic syndrome.

Materials and methods: A total of 109 obese children and adolescents and 44 healthy and lean control subjects were enrolled in the study. According to their ultrasonographic steatosis scores, they were classified into four groups as follows: healthy children; obese, non-NAFLD; obese, grade I-NAFLD; and obese, grade II–III NAFLD. The biochemical parameters and insulin resistance (HOMA-IR) were evaluated from fasting samples. The plasma total antioxidant status (TAS), total oxidant status (TOS), and serum paraoxonase activities were measured and then oxidative stress index (OSI) was calculated as the indicator of degree of oxidative stress.

Results: As the steatosis increased, the alanine aminotransferase, C-reactive protein, HOMA-IR, total cholesterol, and LDL cholesterol increased, whereas HDL cholesterol decreased. The TAS measurements were higher in the obese NAFLD group compared with that of the healthy control group. The TOS and OSI measurements did not differ between the groups. Paraoxonase activities increased significantly as steatosis increased.

Conclusions: Among the children in this study, no relationship could be demonstrated between obesity with/without steatosis and oxidant/antioxidant status.

Keywords: hepatosteatosis; paraoxonase; total antioxidant stress; total oxidant stress

References

  • 1.

    Kim CH, Younossi ZM. Nonalcoholic fatty liver disease: a manifestation of the metabolic syndrome. Cleve Clin J Med 2008;75:721–8.PubMedCrossrefGoogle Scholar

  • 2.

    Pagano G, Pacini G, Musso G, Gambino R, Mecca F, et al. Nonalcoholic steatohepatitis, insulin resistance, and metabolic syndrome: further evidence for an etiological association. Hepatology 2002;35:367–72.CrossrefGoogle Scholar

  • 3.

    Baskol G, Baskol M, Kocer D. Oxidative stress and and antioxidant defences in serum of patients with non-alcoholic steatohepatitis. Clin Biochem 2007;40:776–80.CrossrefWeb of ScienceGoogle Scholar

  • 4.

    Misra A, Khurana L. Obesity and the metabolic syndrome in developing countries. J Clin Endoc Metab 2008;93:9–30.CrossrefWeb of ScienceGoogle Scholar

  • 5.

    Mackness MI, Mackness B, Durrigton PN. Paraoxonase and coronary heart disease. Atherosclerosis 2002;3:49–55.CrossrefGoogle Scholar

  • 6.

    Camps J, Marsillach J, Joven J. The paraoxonases: role in human diseases and methodological difficulties in measurement. Crit Rev Clin Lab Sci 2009;46:83–106.PubMedCrossrefWeb of ScienceGoogle Scholar

  • 7.

    Bhattacharyya T, Nicholls SJ, Topol EJ, Zhang R, Yang X, et al. Relationship of paraoxonase 1 (PON1) gene polymorphisms and functional activity with systemic oxidative sterss and cardiovascular risk. J Am Med Assoc 2008;299:1265–76.Web of ScienceGoogle Scholar

  • 8.

    Koncsos P, Seres I, Harangi M, Illyes I, Jozsa L, et al. Human paraoxonase-1 activity in childhood obesity and its relation to leptin and adiponectin levels. Pediatr Res 2010;67:309–13.Web of ScienceCrossrefGoogle Scholar

  • 9.

    Li HL, Liu DP, Liang CC. Paraoxonase gene polymorphisms, oxidative stress and diseases. J Mol Med 2003;81:766–79.PubMedCrossrefGoogle Scholar

  • 10.

    Barlow SE and the Expert Committee. Expert committee recommendations regarding the prevention, assessment, and treatment of child and adolescent overweight and obesity: summary report. Paediatrics 2007;120:164–92.CrossrefGoogle Scholar

  • 11.

    National High Blood Pressure Education Programme Working Group on High Blood Pressure in Children and Adolescents. The fourth report on the diagnosis, evaluation, and treatment of high blood pressure in children and adolescents. Paediatrics 2004;114:555–76.Google Scholar

  • 12.

    Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, et al. Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985;28:412–9.PubMedCrossrefGoogle Scholar

  • 13.

    Valerio G, Licenziati MR, Iannuzzi A, Franzese A, Siani P, et al. Insulin resistance and impaired glucose tolerance in obese children and adolescents from Southern Italy. Nutr Metab Cardiovasc Dis 2006;16:279–84.PubMedCrossrefGoogle Scholar

  • 14.

    Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. Executive summary of the third report of the National Cholesterol Education Programme (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). Circulation 2002;106:3143–421.Google Scholar

  • 15.

    Kim SH, Lee JM, Kim JH, Kim KG, Han JK, et al. Appropriateness of a donor liver with respect to macrosteatosis: application of artificial neural networks to US images- initial experience. Radiology 2005;234:793–803.Google Scholar

  • 16.

    Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation more stable ABTS radical cation. Clin Biochem 2004;37:277–85.PubMedCrossrefGoogle Scholar

  • 17.

    Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005;38:1103–11.CrossrefPubMedGoogle Scholar

  • 18.

    Kosecik M, Erel O, Sevinc E, Selek S. Increased oxidative stress in children exposed to passive smoking. Int J Cardiol 2005;100:61–4.CrossrefPubMedGoogle Scholar

  • 19.

    Eckerson HW, Wyte MC, La Du BN.The human serum paraoxonase/arylesterase polymorphism. Am J Hum Genet 1083;35:1126–38.Google Scholar

  • 20.

    Yang S, Zhu H, Li Y, Lin H, Gabrielson K, et al. Mitochondrial adaptations to obesity-related oxidant stress. Arch Biochem Biophys 2000;378:259–68.Google Scholar

  • 21.

    Videla LA, Rodrigo R, Orenella M, Fernandez V, Tapia G, et al. Oxidative stress-related parameters in the liver of non-alcoholic fatty liver disease patients. Clin Sci (Lond) 2004;106:261–68.CrossrefPubMedGoogle Scholar

  • 22.

    Koruk M, Taysi S, Savas MC, Yılmaz O, Akçay F, et al. Oxidative stress and enzymatic status in patients with nonalcoholic steatohepatitis. Ann Clin Lab Sci 2004;34:57–62.PubMedGoogle Scholar

  • 23.

    Nobili V, Manco M, Devito R, Di Ciommo V, Comparcola D, et al. Lifestyle intervention and antioxidant therapy in children with nonalcoholic fatty liver disease: a randomized, controlled trial. Hepatology 2008:48:119–28.Web of ScienceCrossrefPubMedGoogle Scholar

  • 24.

    Pirgon O, Bilgin H, Cekmez F, Kurku H, Dundar BN. Association between insulin resistance and oxidative stress parameters in obese adolescents with non-alcoholic fatty liver disease. J Clin Res Pediatr Endocrinol 2013;5:33–9.PubMedGoogle Scholar

  • 25.

    Krystek-Korpacka M, Patryn E, Hotowy K, Czapinska E, Majda J, et al. Paraoxonase (PON)-1 activity in overweight and obese children and adolescents: association with obesity-related inflammation and oxidative stress. Adv Clin Exp Med 2013;22:229–36.Google Scholar

  • 26.

    Sumegova K, Nagyova Z, Waczulıkova I, Zıtnanova I, Durackova Z. Activity of paraoxonase 1 and lipid profile in healthy children. Physiol Res 2007;56:351–7.Google Scholar

  • 27.

    Tomas M, Senti M, Garcia-Faria F, Vila J, Torrents A, et al. Effect of simvastatin therapy on paraoxonase activity and related lipoproteins in familial hypercholesterolemic patients. Arteroscler Thromb Vasc Biol 2000;20:2113–9.CrossrefGoogle Scholar

  • 28.

    Whaley-Connell A, Mc Cullough PA, Sowers JR. The role of oxidative stress in metabolic syndrome. Rev Cardiovasc Med 2011;12:21–9.Google Scholar

  • 29.

    Tabur S, Torun AN, Sabuncu T, Turan MN, Celik H, et al. Non-diabetic metabolic syndrome and obesity do not affect serum paraoxonase and arylesterase activities but do affect oxidative stress and inflammation. Eur J Endocrinol 2010;162:535–41.Web of SciencePubMedCrossrefGoogle Scholar

  • 30.

    Hansel B, Giral P, Nobecourt E, Chantepie S, Bruckert E, et al. Metabolic syndrome is associated with elevated oxidative stress and dysfunctional dense high density lipoprotein particles displaying impaired antioxidative activity. J Clin Endocrinol Metab 2004;89:4963–71.CrossrefPubMedGoogle Scholar

  • 31.

    Senti M, Tomas M, Fito M, Weinbrenner T, Covas MI, et al. Antioxidant paraoxonase 1 activity in metabolic syndrome. J Clin Endocrinol Metab 2003;88:1637–46.Google Scholar

  • 32.

    Ferretti G, Bacchetti T, Moroni C, Savino S, Liuzzi A, et al. Paraoxonase activity in high-density lipoproteins: a comparition between healthy and obese females. J Clin Endocrinol Metab 2005;90:1728–33.CrossrefGoogle Scholar

  • 33.

    Molnar D, Decsi T, Koletzko B. Reduced antioxidant status in obese children with multimetabolic syndrome. Int J Obes Relat Metab Disord 2004;28:1197–202.PubMedCrossrefGoogle Scholar

  • 34.

    Weghuber D, Roden M, Franz C, Chmelik M, Torabia S, et al. Vascular function in obese children with non-alcoholic fatty liver disease. Int J Pediatr Obes 2011;6:120–7.PubMedCrossrefGoogle Scholar

  • 35.

    Goswami B, Tayal D, Gupta N, Mallika V. Paraoxonase: a multifaceted biomolecule. Clinica Chimica Acta 2009;410: 1–12.Web of ScienceGoogle Scholar

  • 36.

    Ruperez AL, Lopez-Guarnido O, Olza J, Gil-Campos M, Leis R, et al. Paraoxonase 1 activities and genetic variation in childhood obesity. Br J Nutr 2013;110:1639–47.Web of SciencePubMedGoogle Scholar

About the article

Corresponding author: Emel Torun, MD, Department of Pediatrics, Bezmialem Vakif University Hospital, Adnan Menderes Avenue, P.K.: 3409 Fatih, Istanbul, Phone: +0090/212 453-1700, Fax: +0090/212 621-7580, E-mail:


Received: 2013-08-20

Accepted: 2014-02-18

Published Online: 2014-03-29

Published in Print: 2014-07-01


Citation Information: Journal of Pediatric Endocrinology and Metabolism, Volume 27, Issue 7-8, Pages 667–675, ISSN (Online) 2191-0251, ISSN (Print) 0334-018X, DOI: https://doi.org/10.1515/jpem-2013-0337.

Export Citation

©2014 by Walter de Gruyter Berlin/Boston.Get Permission

Citing Articles

Here you can find all Crossref-listed publications in which this article is cited. If you would like to receive automatic email messages as soon as this article is cited in other publications, simply activate the “Citation Alert” on the top of this page.

[1]
Raluca Sandor, Daniel Leucuta, Eleonora Dronca, Alexandru Niculae, Victoria Cret, Ciprian Silaghi, and Soimita Suciu
Revista Romana de Medicina de Laborator, 2015, Volume 23, Number 4
[2]
Reza Fadaei, Reza Meshkani, Hossein Poustchi, Soudabeh Fallah, Nariman Moradi, Ghodratollah Panahi, Shahin Merat, and Taghi Golmohammadi
Archives of Physiology and Biochemistry, 2018, Page 1
[3]
Camila TC de Lira, Marcos AM dos Santos, Priscyla P Gomes, Yara L Fidelix, Ana CO dos Santos, Thiago RS Tenório, Mara C Lofrano-Prado, and Wagner L do Prado
Nutrition and Health, 2017, Volume 23, Number 4, Page 281
[4]
Grażyna Rowicka, Hanna Dyląg, Jadwiga Ambroszkiewicz, Agnieszka Riahi, Halina Weker, and Magdalena Chełchowska
Oxidative Medicine and Cellular Longevity, 2017, Volume 2017, Page 1
[5]
Bulent Demir, Esra Demir, Gonul Acıksarı, Turgut Uygun, Irem Kırac Utku, Asuman Gedikbasi, Ilker Murat Caglar, Osman Pirhan, Hande Oktay Tureli, Ersan Oflar, İsmail Ungan, Serkan Ciftci, and Osman Karakaya
International Journal of Endocrinology, 2014, Volume 2014, Page 1

Comments (0)

Please log in or register to comment.
Log in