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Publicly Available Published by De Gruyter June 24, 2012

Structural and functional impairments of polysialic acid (polySia)-neural cell adhesion molecule (NCAM) synthesized by a mutated polysialyltransferase of a schizophrenic patient

Masaya Hane, Mizuki Sumida, Ken Kitajima and Chihiro Sato

Polysialic acid (polySia) is a homopolymer of sialic acid with a degree of polymerization (DP) of 8–400. When present on neural cell adhesion molecule (NCAM), polySia has anti-adhesive effects on cell–cell interactions owing to its bulky polyanionic nature, and is involved in the regulation of neurogenesis and neuronal functions. Recently, we demonstrated that polySia functions not only as an anti-cell adhesion molecule, but also as a reservoir scaffold for brain-derived neurotrophic factor (BDNF) and fibroblast growth factor 2 (FGF2), which are biologically active molecules in neurogenesis. To understand the significance of polySia structure in the reservoir function, we focused on polySia-NCAM biosynthesized by mutated polysialyltransferase (ST8SiaII or STX) that was reported in a schizophrenia patient. The polySia-NCAM biosynthesized by mutant ST8SiaII/STX contained less polySia with shorter chain length and exhibited impaired reservoir function for BDNF and FGF2 as compared with that synthesized by wild-type (wt) ST8SiaII/STX. Our findings suggest that the quantity and quality of polySia on NCAM are important for normal neuronal functioning.


Conference

IUPAC Congress, IUPAC Congress, CONGRESS, IUPAC Congress, 43rd, San Juan, Puerto Rico, 2011-07-30–2011-08-07


References

1 F. A. Troy II. “Sialobiology and the polysialic acid glycotope”, in Biology of the Sialic Acids, A. Rosenberg (Ed.), p. 95, Plenum, New York (1996).10.1007/978-1-4757-9504-2_4Search in Google Scholar

2 10.4052/tigg.11.371, C. Sato, K. Kitajima. Trends Glycosci. Glycotechnol.11, 371 (1999).Search in Google Scholar

3 10.1074/jbc.M508762200, D. Nakata, F. A. Troy II. J. Biol. Chem.280, 38305 (2005).Search in Google Scholar

4 10.1016/j.pneurobio.2006.08.003, L. Bonfanti. Prog. Neurobiol.80, 129 (2006).Search in Google Scholar

5 10.1038/nrn2285, U. Rutishauser. Nat. Rev. Neurosci.9, 26 (2008).Search in Google Scholar

6 10.1016/S0168-0102(09)80003-5, T. Seki, Y. Arai. Neurosci. Res.12, 503 (1991).Search in Google Scholar

7 T. Seki, Y. Arai. J. Neurosci.13, 2351 (1993).Search in Google Scholar

8 10.1016/0168-0102(93)90111-3, T. Seki, Y. Arai. Neurosci. Res.17, 265 (1993).Search in Google Scholar

9 10.1038/367455a0, H. Cremer, R. Lange, A. Christoph, M. Plomann, G. Vopper, J. Roes, R. Brown, S. Baldwin, P. Kraemer, S. Scheff. Nature367, 455 (1994).Search in Google Scholar PubMed

10 M. Eckhardt, O. Bukalo, G. Chazal, L. Wang, C. Goridis, M. Schachner, R. Gerardy-Schahn, H. Cremer, A. Dityatev. J. Neurosci.20, 5234 (2000).Search in Google Scholar

11 10.1074/jbc.M403429200, K. Angata, J. Long, O. Bukalo, W. Lee, A. Dityatev, A. Wynshaw-Boris, M. Schachner, M. Fukuda, J. Marth. J. Biol. Chem.279, 32603 (2004).Search in Google Scholar PubMed

12 10.1093/glycob/cwn084, Y. Kanato, K. Kitajima, C. Sato. Glycobiology18, 1044 (2008).Search in Google Scholar PubMed

13 10.1271/bbb.90637, Y. Kanato, S. Ono, K. Kitajima, C. Sato. Biosci. Biotechnol. Biochem.73, 2735 (2009).Search in Google Scholar

14 10.1074/jbc.M111.221143, R. Isomura, K. Kitajima, C. Sato. J. Biol. Chem.286, 21535 (2011).Search in Google Scholar

15 10.1074/jbc.M111.276618, S. Ono, M. Hane, K. Kitajima, C. Sato. J. Biol. Chem.287, 3710 (2012).Search in Google Scholar

16 10.1038/sj.mp.4000784, J. Millar, S. Christie, S. Anderson, D. Lawson, D. Hsiao-Wei Loh, R. Devon, B. Arveiler, W. Muir, D. Blackwood, D. Porteous Mol. Psychiatry6, 173 (2001).Search in Google Scholar

17 10.1007/s00213-003-1761-y, P. Harrison. Psychopharmacology (Berlin)174, 151 (2004).Search in Google Scholar

18 10.1016/S0165-1781(96)03111-3, R. Strous, N. Bark, S. Parsia, J. Volavka, H. Lachman. Psychiatry Res.69, 71 (1997).Search in Google Scholar

19 10.1038/ng.171, N. Allen, S. Bagade, M. McQueen, J. Ioannidis, F. Kavvoura, M. Khoury, R. Tanzi, L. Bertram. Nat. Genet.40, 827 (2008).Search in Google Scholar

20 10.1016/S0074-7742(06)78012-6, O. Guillin, C. Demily, F. Thibaut. Int. Rev. Neurobiol.78, 377 (2007).Search in Google Scholar

21 10.1159/000110430, U. Lang, I. Puls, D. Muller, N. Strutz-Seebohm, J. Gallinat. Cell Physiol. Biochem.20, 687 (2007).Search in Google Scholar PubMed

22 B. Woods. Am. J. Psychiatry155, 1661 (1998).10.1176/ajp.155.12.1661Search in Google Scholar PubMed

23 10.1016/S0278-5846(01)00159-2, P. Ashe, M. Berry, A. Boulton. Prog. Neuropsychopharmacol. Biol. Psychiatry25, 691 (2001).Search in Google Scholar

24 10.1073/pnas.92.7.2785, D. Barbeau, J. Liang, Y. Robitalille, R. Quirion, L. Srivastava. Proc. Natl. Acad. Sci. USA92, 2785 (1995).Search in Google Scholar

25 10.1001/archpsyc.60.12.1193, B. Turetsky, P. Moberg, D. Roalf, S. Arnold, R. Gur. Arch. Gen. Psychiatry60, 1193 (2003).Search in Google Scholar

26 10.1038/sj.mp.4001594, M. Maziade, M. Roy, Y. Chagnon, D. Cliche, J. Fournier, N. Montgrain, C. Dion, J. Lavallée, Y. Garneau, N. Gingras, L. Nicole, A. Pirès, A. Ponton, A. Potvin, H. Wallot, C. Mérette. Mol. Psychiatry10, 486 (2005).Search in Google Scholar

27 10.1016/j.biopsych.2005.08.016, M. Arai, K. Yamada, T. Toyota, N. Obata, S. Haga, Y. Yoshida, K. Nakamura, Y. Minabe, H. Ujike, I. Sora, K. Ikeda, N. Mori, T. Yoshikawa, M. Itokawa. Biol. Psychiatry59, 652 (2006).Search in Google Scholar

28 10.1006/abio.1998.2718, C. Sato, S. Inoue, T. Matsuda, K. Kitajima. Anal. Biochem.261, 191 (1998).Search in Google Scholar

29 C. Sato, S. Inoue, T. Matsuda, K. Kitajima. Anal. Biochem.262, (1999).Search in Google Scholar

30 10.1016/S0076-6879(10)78010-1, C. Sato, N. Yamakawa, K. Kitajima. Methods Enzymol.478, 219 (2010).Search in Google Scholar

31 10.4052/tigg.23.221, C. Sato, K. Kitajima. Trends Glycosci. Glycotechnol.23, 221 (2011).Search in Google Scholar

32 10.1074/jbc.M010542200, A. K. Datta, R. Chammas, J. C. Paulson. J. Biol. Chem.276, 15200 (2001).Search in Google Scholar PubMed

33 10.1073/pnas.0912103107, S. P. Galuska, M. Rollenhagen, M. Kaup, K. Eggers, I. Oltmann-Norden, M. Schiff, M. Hartmann, B. Weinhold, H. Hildebrandt, R. Geyer, M. Mühlenhoff, H. Geyer. Proc. Natl. Acad. Sci. USA107, 10250 (2010).Search in Google Scholar PubMed PubMed Central

34 10.1126/science.1072356, T. Biederer, Y. Sara, M. Mozhayeva, D. Atasoy, X. Liu, E. T. Kavalali, T. C. Südhof. Science297, 1525 (2002).Search in Google Scholar PubMed

35 10.1074/jbc.M111.322024, J. L. Zapater, K. J. Colley. J. Biol. Chem.287, 6441 (2012).Search in Google Scholar PubMed PubMed Central

36 K. Murphy, A. Foley, A. O’Connell, C. Regan. Neuropsychopharmacology31, 90 (2006).10.1038/sj.npp.1300810Search in Google Scholar PubMed

37 R. Miñana, E. Climent, D. Barettino, J. M. Segui, J. Renau-Piqueras, C. J. Guerri. Neurochemistry75, 954 (2000).10.1046/j.1471-4159.2000.0750954.xSearch in Google Scholar PubMed

38 10.1002/jnr.10781, M. Brocco, G. Pollevick, A. Frasch. J. Neurosci. Res.74, 744 (2003).Search in Google Scholar PubMed

39 10.1002/ana.20506, K. Yoshimi, Y. Ren, T. Seki, M. Yamada, H. Ooizumi, M. Onodera, Y. Saito, S. Murayama, H. Okano, Y. Mizuno, H. Mochizuki. Ann. Neurol.58, 31 (2005).Search in Google Scholar PubMed

Online erschienen: 2012-6-24
Erschienen im Druck: 2012-6-24

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