In this chapter, the economic framework and action points for the upscaling and commercialization of biotechnological processes as well as product designs will be examined with a special focus on autotrophic processes. The most important factors influencing the evaluation of processes and the investment readiness will be summarized. Besides other factors, the regulatory framework, sustainability, the unique selling point, chances and barriers will be described with a special emphasis on autotrophic processes. The need and benefit of using defined technology readiness levels will be introduced. The technological challenges of autotrophic processes in particular will be described including required implementation steps, such as devices and downstream processing technologies.