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Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

A Non-Radioactive Method for Inexpensive Quantitative RT-PCR

M. Maggiolini, O. Donzé and D. Picard
From the journal Biological Chemistry

Abstract

We present a novel method for quantitative RT-PCR that involves direct incorporation of digoxigenin-11-dUTP (DIG-dUTP) during amplification of cDNAs, separation of RT-PCR products by agarose gel electrophoresis, Southern transfer to a nylon membrane, and chemiluminescent detection with an anti-DIG antibody. The whole procedure can be done in about a day and has the following advantages: It is highly sensitive, specificity is confirmed by monitoring the size of the RT-PCR product, it is non-radioactive, quantitative, and does not require expensive specialized equipment.

Published Online: 2005-6-1
Published in Print: 1999-6-1

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

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