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Licensed Unlicensed Requires Authentication Published by De Gruyter July 5, 2005

Identification of the Arabidopsis thaliana Flavonoid 3'-Hydroxylase Gene and Functional Expression of the Encoded P450 Enzyme

  • C. Schoenbohm , S. Martens , C. Eder , G. Forkmann and B. Weisshaar
From the journal Biological Chemistry

Abstract

The phenylpropanoid pathway results in the synthesis of thousands of compounds, including flavonoids like flavonols, anthocyanidins and tannins. In Arabidopsis thaliana, the lack of tannins in the seed coat (testa) causes the transparent testa (tt) phenotype. In the present study, we identified the gene responsible for the tt7 mutation. We show that TT7 encodes the enzyme flavonoid 3′-hydroxylase (F3′H), and demonstrate that this P450-dependent monooxygenase has F3′H activity. The availability of the AtF3′H gene and promoter sequence will allow us to study the coregulation of a complete set of flavonol and anthocyanidin biosynthesis genes in A. thaliana, and makes in vitro synthesis of hydroxylated flavonoids more feasible.

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Published Online: 2005-07-05
Published in Print: 2000-08-06

Copyright © 2000 by Walter de Gruyter GmbH & Co. KG

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