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Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Generation of glycogen- and albumin-producing hepatocyte-like cells from embryonic stem cells

  • Gabriela Kania , Przemyslaw Blyszczuk , Andrea Jochheim , Michael Ott and Anna M. Wobus
From the journal Biological Chemistry


We present a novel two-step protocol for the differentiation of embryonic stem (ES) cells into the hepatic lineage. Differentiated hepatocyte-like cells express genes and proteins characteristic for endodermal and hepatic cells and acquire a functional hepatic phenotype as demonstrated by albumin secretion and glycogen storage. During differentiation, α-fetoprotein, albumin, transthyretin, α-1-antitrypsin, cytochrome P450 subunits 2b9 and 2b13 and tyrosine aminotransferase transcripts are upregulated. Quantitative RT-PCR data revealed a fetal hepatic phenotype corresponding to day 13–14 of liver development. Terminally differentiated hepatocyte-like cells show a bi-nucleated, cuboidal morphology labeled by albumin, α-1-antitrypsin, liver amylase, dipeptidyl peptidase IV, c-met and cytokeratin 18.

ES-derived intermediate cell types transiently and partially co-express nestin with albumin and α-fetoprotein, respectively, but not cytokeratin 19. This finding suggests an ES-derived potential hepatic progenitor cell type, which is partially nestin-, albumin- and α-fetoproteinpositive, but cytokeratin 19-negative.



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Published Online: 2005-06-01
Published in Print: 2004-10-01

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