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Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Developments in Quantitative PCR

  • Claudio Orlando , Pamela Pinzani and Mario Pazzagli
From the journal


In recent years the growing interest in quantitative applications of the polymerase chain reaction (PCR) has favoured the development of a large number of assay procedures suitable for this purpose. In this paper we review some basic principles of quantitative PCR and in particular the role of reference materials and calibrators and the different strategies adopted for nucleic acid quantification. We focus on two methodological approaches for quantitative PCR in this review: competitive PCR and real-time quantitative PCR based on the use of fluorogenic probes. The first is one of the most common methods of quantitative PCR and we discuss the structure of the competitors and the various assay procedures. The second section is dedicated to a recent promising technology for quantitative PCR in which the use of fluorogenic probes and dedicated instrumentation allows the development of homogeneous methods. Assay performance of these methods in terms of practicability and reliability indicates that these kinds of technologies will have a widespread use in the clinical laboratory in the near future.

Published Online: 2005-06-01
Published in Print: 1998-05-01

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

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