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Licensed Unlicensed Requires Authentication Published by De Gruyter June 1, 2005

Quantitation and Enzymatic Activity of the Alkaline Protease from Pseudomonas aeruginosa in Culture Supernatants from Clinical Strains

  • Dominique Louis , Pierre Sorlier and Jean Wallach
From the journal


Alkaline protease from Pseudomonas aeruginosa (E.C., also called pseudomonal serralysin, plays an important role in the infection bacterial process. The alkaline protease concentration and activity in the culture supernatants of 23 Pseudomonas aeruginosa strains from patients suffering from cystic fibrosis were determined by using an ELISA procedure and a specific enzymatic activity test. No correlation was demonstrated between the activity and the concentration of alkaline protease. This could be explained by the presence of an inactive precursor and a partial hydrolysis of the enzyme, confirmed by SDS-PAGE and Western blotting in the supernatants. The activity test is the only one allowing a quantification of the presence of an active form of alkaline protease.

Published Online: 2005-06-01
Published in Print: 1998-05-01

Copyright © 1999 by Walter de Gruyter GmbH & Co. KG

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